PMID- 25653381
OWN - NLM
STAT- MEDLINE
DCOM- 20150414
LR  - 20181113
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 35
IP  - 5
DP  - 2015 Feb 4
TI  - Activity-dependent rapid local RhoA synthesis is required for hippocampal 
      synaptic plasticity.
PG  - 2269-82
LID - 10.1523/JNEUROSCI.2302-14.2015 [doi]
AB  - Dendritic protein synthesis and actin cytoskeleton reorganization are important 
      events required for the consolidation of hippocampal LTP and memory. However, the 
      temporal and spatial relationships between these two processes remain unclear. 
      Here, we report that treatment of adult rat hippocampal slices with BDNF or with 
      tetraethylammonium (TEA), which induces a chemical form of LTP, produces a rapid 
      and transient increase in RhoA protein levels. Changes in RhoA were restricted to 
      dendritic spines of CA3 and CA1 and require de novo protein synthesis regulated 
      by mammalian target of rapamycin (mTOR). BDNF-mediated stimulation of RhoA 
      activity, cofilin phosphorylation, and actin polymerization were completely 
      suppressed by protein synthesis inhibitors. Furthermore, intrahippocampal 
      injections of RhoA antisense oligodeoxynucleotides inhibited theta burst 
      stimulation (TBS)-induced RhoA upregulation in dendritic spines and prevented LTP 
      consolidation. Addition of calpain inhibitors after BDNF or TEA treatment 
      maintained RhoA levels elevated and prolonged the effects of BDNF and TEA on 
      actin polymerization. Finally, the use of isoform-selective calpain inhibitors 
      revealed that calpain-2 was involved in RhoA synthesis, whereas calpain-1 
      mediated RhoA degradation. Overall, this mechanism provides a novel link between 
      dendritic protein synthesis and reorganization of the actin cytoskeleton in 
      hippocampal dendritic spines during LTP consolidation.
CI  - Copyright (c) 2015 the authors 0270-6474/15/352269-14$15.00/0.
FAU - Briz, Victor
AU  - Briz V
AD  - Graduate College of Biomedical Sciences and.
FAU - Zhu, Guoqi
AU  - Zhu G
AD  - Graduate College of Biomedical Sciences and.
FAU - Wang, Yubin
AU  - Wang Y
AD  - Graduate College of Biomedical Sciences and.
FAU - Liu, Yan
AU  - Liu Y
AD  - Graduate College of Biomedical Sciences and College of Osteopathic Medicine of 
      the Pacific Western University of Health Sciences, Pomona, California 91766.
FAU - Avetisyan, Mariam
AU  - Avetisyan M
AD  - Graduate College of Biomedical Sciences and.
FAU - Bi, Xiaoning
AU  - Bi X
AD  - College of Osteopathic Medicine of the Pacific Western University of Health 
      Sciences, Pomona, California 91766.
FAU - Baudry, Michel
AU  - Baudry M
AD  - Graduate College of Biomedical Sciences and mbaudry@westernu.edu.
LA  - eng
GR  - R15 MH101703/MH/NIMH NIH HHS/United States
GR  - P01NS045260-01/NS/NINDS NIH HHS/United States
GR  - P01 NS045260/NS/NINDS NIH HHS/United States
GR  - R01NS057128/NS/NINDS NIH HHS/United States
GR  - R01 NS057128/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Actin Depolymerizing Factors)
RN  - 0 (Actins)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 66-40-0 (Tetraethylammonium)
RN  - EC 3.4.22.- (Calpain)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Actin Depolymerizing Factors/metabolism
MH  - Actins/metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - CA1 Region, Hippocampal/drug effects/*metabolism/physiology
MH  - CA3 Region, Hippocampal/drug effects/*metabolism/physiology
MH  - Calpain/antagonists & inhibitors/metabolism
MH  - Dendritic Spines/metabolism
MH  - *Long-Term Potentiation
MH  - Male
MH  - Organ Specificity
MH  - Phosphorylation
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Proteolysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tetraethylammonium/pharmacology
MH  - rhoA GTP-Binding Protein/genetics/*metabolism
PMC - PMC4315844
OTO - NOTNLM
OT  - BDNF
OT  - LTP
OT  - actin cytoskeleton
OT  - calpain
OT  - dendritic protein synthesis
COIS- The authors declare no competing financial interests.
EDAT- 2015/02/06 06:00
MHDA- 2015/04/15 06:00
PMCR- 2015/08/04
CRDT- 2015/02/06 06:00
PHST- 2015/02/06 06:00 [entrez]
PHST- 2015/02/06 06:00 [pubmed]
PHST- 2015/04/15 06:00 [medline]
PHST- 2015/08/04 00:00 [pmc-release]
AID - 35/5/2269 [pii]
AID - 2302-14 [pii]
AID - 10.1523/JNEUROSCI.2302-14.2015 [doi]
PST - ppublish
SO  - J Neurosci. 2015 Feb 4;35(5):2269-82. doi: 10.1523/JNEUROSCI.2302-14.2015.