PMID- 25653756
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150205
LR  - 20200930
IS  - 2008-8140 (Print)
IS  - 2322-3618 (Electronic)
IS  - 2008-8140 (Linking)
VI  - 3
IP  - 2
DP  - 2012 Spring
TI  - Fibroblast and T cells conditioned media induce maturation dendritic cell and 
      promote T helper immune response.
PG  - 111-8
AB  - Dendritic cells (DCs) induce pathogen-specific T cell responses. We 
      comprehensively studied the effects of addition of maturation stimulus, 
      fibroblasts (fibroblast conditioned medium), PHA activated T cells (T cell 
      conditioned medium), and mixture of fibroblast & PHA activated T cells (FCM-TCCM) 
      conditioned media on maturation of DCs. Monocytes were cultured with GM-CSF and 
      IL-4 for five days. Maturation factors included MCM and TNF-alpha as control group. 
      FCM and TCCM, or FCM-TCCM supernatant were considered as the treatment group. 
      Tumor antigens were added at day five. Matured DCs were harvested at day seven. 
      Phenotypic and functional analyses were carried out using anti (CD14, CD80, CD86, 
      CD83 and HLA-DR) monoclonal antibodies. Phagocytic activity, mixed lymphocyte 
      reaction (MLR) and cytokine production were also evaluated. At the end of 
      culturing period, significantly fully matured DCs with large amount cytoplasm and 
      copious dendritic projections were found in the presence of MCM, TNF-alpha with or 
      without FCM, TCCM, FCM as well as TCCM. Flow cytometric analysis revealed that 
      expression of CD14 decreased in particular in treated DCs, at the 5(th) day and 
      expression of CD80, CD86 and HLA-DR was higher when FCM, TCCM, FCM plus TCCM were 
      added to maturation factor. This study demonstrated that DCs matured with these 
      methods had optimum function in comparison with either factor alone.
FAU - Asadi, Masoumeh
AU  - Asadi M
AD  - Department of Biology and Embryology, Faculty of Science, Urmia University, 
      Urmia, Iran;
FAU - Farokhi, Farah
AU  - Farokhi F
AD  - Department of Biology and Embryology, Faculty of Science, Urmia University, 
      Urmia, Iran;
FAU - Delirezh, Nowruz
AU  - Delirezh N
AD  - Department of Immunology, Faculty of Veterinary Medicine, Urmia University, 
      Urmia, Iran.
FAU - Ganji Bakhsh, Meysam
AU  - Ganji Bakhsh M
AD  - Department of Biology and Embryology, Faculty of Science, Urmia University, 
      Urmia, Iran;
FAU - Nejati, Vahid
AU  - Nejati V
AD  - Department of Biology and Embryology, Faculty of Science, Urmia University, 
      Urmia, Iran;
FAU - Golami, Keykavos
AU  - Golami K
AD  - Department of Biology and Embryology, Faculty of Science, Urmia University, 
      Urmia, Iran;
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Vet Res Forum
JT  - Veterinary research forum : an international quarterly journal
JID - 101625812
PMC - PMC4312805
OTO - NOTNLM
OT  - Dendritic cell
OT  - Fibroblast
OT  - T cell
EDAT- 2012/04/01 00:00
MHDA- 2012/04/01 00:01
PMCR- 2012/03/01
CRDT- 2015/02/06 06:00
PHST- 2011/10/26 00:00 [received]
PHST- 2012/02/27 00:00 [revised]
PHST- 2012/06/15 00:00 [accepted]
PHST- 2015/02/06 06:00 [entrez]
PHST- 2012/04/01 00:00 [pubmed]
PHST- 2012/04/01 00:01 [medline]
PHST- 2012/03/01 00:00 [pmc-release]
AID - vrf-3-111 [pii]
PST - ppublish
SO  - Vet Res Forum. 2012 Spring;3(2):111-8.