PMID- 25654284
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20181202
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 22
IP  - 12
DP  - 2014 Dec
TI  - [In vivo tracking of PKH26-labeled human umbilical cord mesenchymal stem cells 
      after transplantation into rats with liver cirrhosis].
PG  - 910-4
LID - cma.j.issn.1007-3418.2014.12 [pii]
LID - 10.3760/cma.j.issn.1007-3418.2014.12.007 [doi]
AB  - OBJECTIVE: To observe the in vivo migration of human umbilical cord mesenchymal 
      stem cells (hUCMSCs) labeled with the PKH26 red fluorescent dye after 
      transplantation into rats with liver cirrhosis. METHODS: Frozen hUCMSCs were 
      resuscitated and labeled with PKH26. Labeling efficiency and fluorescent 
      maintenance time of the PKH26-1abeled cells were measured. Morphology of the 
      labeled and unlabeled (control) cells was observed by microscopy. The cell growth 
      curve was determined using the MTT method. The PKH26-1abeled hUCMSCs were 
      transplanted via tail vein injection into healthy (control) rats and rats with 
      liver cirrhosis. Migration of the PKH26-1abeled hUCMSCs observed 48 h later in 
      frozen liver sections under a fluorescence microscope. RESULTS: The labeling 
      ratio of PKH26 to hUCMSCs was 100%. Growth of the labeled cells was good. The 
      cell morphology was not significantly different between the labeled and unlabeled 
      cells; all cells were long and spindle-like. Cell proliferation was not impacted 
      significantly by labeling.Fluorescence was maintained for at least 20 days, as 
      detected by in vitro analysis. After transplantation into the rats, the 
      PKH26-1abeled hUCMSCs were mainly distributed in the area surrounding the portal 
      vein, the blood vessels, and the false lobule of the cirrhotic liver; a small 
      amount ofhUCMSCs were present in the spleen and lung. CONCLUSION: PKH26 is an 
      ideal fluorescent dye to label hUCMSCs. The PKH26 labeling technique can be used 
      to study the migration of hUCMSCs in cirrhotic liver.
FAU - Xue, Gai
AU  - Xue G
AD  - Department of Pharmacology, Bethune International Peace Hospital, Shijiazhuang 
      050082, China.
FAU - Li, Jianli
AU  - Li J
FAU - Liu, Jianfang
AU  - Liu J
FAU - Wu, Honghai
AU  - Wu H
FAU - Hou, Yanning
AU  - Hou Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Organic Chemicals)
RN  - 0 (PKH 26)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Fluorescent Dyes
MH  - Humans
MH  - *Liver Cirrhosis
MH  - *Mesenchymal Stem Cells
MH  - Organic Chemicals
MH  - Rats
MH  - *Umbilical Cord
EDAT- 2015/02/06 06:00
MHDA- 2015/09/09 06:00
CRDT- 2015/02/06 06:00
PHST- 2015/02/06 06:00 [entrez]
PHST- 2015/02/06 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
AID - cma.j.issn.1007-3418.2014.12 [pii]
AID - 10.3760/cma.j.issn.1007-3418.2014.12.007 [doi]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2014 Dec;22(12):910-4. doi: 
      10.3760/cma.j.issn.1007-3418.2014.12.007.