PMID- 25654290
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20181202
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 22
IP  - 12
DP  - 2014 Dec
TI  - [Association of intestinal epithelial tight junctions, E.coli, intestinal mucosa 
      Toll-like receptor-4 and tumor necrosis factor-alpha in rabbit with non-alcoholic 
      fatty liver disease].
PG  - 942-7
LID - cma.j.issn.1007-3418.2014.12 [pii]
LID - 10.3760/cma.j.issn.1007-3418.2014.12.013 [doi]
AB  - OBJECTIVE: To explore the variation and association of intestinal epithelial 
      tight junctions, E.coli, and expression of Toll-like receptor-4 (TLR-4) and tumor 
      necrosis factor-alpha (TNFalpha) in a rabbit model of non-alcoholic fatty liver 
      disease (NAFLD). METHODS: Fourteen male New Zealand rabbits were randomly divided 
      into two groups. The control group was given a normal diet, while the NAFLD group 
      was given a high-fat diet. After 12 weeks, the two groups were sacrificed. Both 
      length and width of the tight junctions of ileum epithelial cells were measured 
      by electron microscopy. Enzyme-linked immunosorbent assay was used to detect the 
      concentration of TNFalpha in venous blood serum. Location and expression of TLR-4 
      were observed by immunohistochemical staining. Copies of E.coli genes were 
      measured by RT-PCR. The relationship between TNFalpha and tight junctions, TLR-4, and 
      E.coli were respectively analyzed by stepwise multiple linear regression. 
      RESULTS: Compared with the control group, the NAFLD group has significantly 
      shortened ileum epithelial fight junctions (t=5.031, P<0.01), increased copies of 
      E. coli genes (t=-3.492, P<0.01), and increased ileal expression of TLR-4 
      (t=-44.089, P<0.01). A significant increase of TNFalpha was also observed in the 
      NAFLD group (t=-17.768, P<0.01). Regression analysis showed that E.coli and 
      intestinal epithelial tight junctions were correlated (standardized beta=-0.385), 
      and showed a positive correlation between E. coli and TLR-4 (standardized 
      beta=0.332 and 0.427). CONCLUSION: Damage to the intestine as a physical barrier, 
      biological barrier and immune barrier is closely related to TNFalpha expression, and 
      the relation between the immune barrier and TNFalpha is more significant.
FAU - Wang, Haikun
AU  - Wang H
AD  - Digestive Department, the First Teaching Hospital of Xinjiang Medical University, 
      Urumqi 830054, China.
FAU - Gao, Hongliang
AU  - Gao H
FAU - Yao, Ping
AU  - Yao P
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - *Epithelial Cells
MH  - *Escherichia coli
MH  - *Intestinal Mucosa
MH  - Male
MH  - *Non-alcoholic Fatty Liver Disease
MH  - Rabbits
MH  - *Tight Junctions
MH  - Toll-Like Receptor 4
MH  - Tumor Necrosis Factor-alpha
EDAT- 2015/02/06 06:00
MHDA- 2015/09/09 06:00
CRDT- 2015/02/06 06:00
PHST- 2015/02/06 06:00 [entrez]
PHST- 2015/02/06 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
AID - cma.j.issn.1007-3418.2014.12 [pii]
AID - 10.3760/cma.j.issn.1007-3418.2014.12.013 [doi]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2014 Dec;22(12):942-7. doi: 
      10.3760/cma.j.issn.1007-3418.2014.12.013.