PMID- 25655391
OWN - NLM
STAT- MEDLINE
DCOM- 20160418
LR  - 20181113
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 38
IP  - 4
DP  - 2015 Aug
TI  - Anti-inflammatory Effects of Aspalathin and Nothofagin from Rooibos (Aspalathus 
      linearis) In Vitro and In Vivo.
PG  - 1502-16
LID - 10.1007/s10753-015-0125-1 [doi]
AB  - Aspalathin (Asp) and nothofagin (Not) are two major active dihydrochalcones found 
      in green rooibos, which have been reported for their anti-oxidant activity. Here, 
      we investigated the anti-inflammatory effects and underlying mechanisms of Asp 
      and Not against lipopolysaccharide (LPS)-mediated vascular inflammatory 
      responses. The anti-inflammatory activities of Asp and Not were determined by 
      measuring permeability, monocytes adhesion and migration, and activation of 
      pro-inflammatory proteins in LPS-activated human umbilical vein endothelial cells 
      (HUVECs) and mice. We found that each compound inhibited LPS-induced barrier 
      disruption, expression of cell adhesion molecules (CAMs), and 
      adhesion/transendothelial migration of neutrophils to human endothelial cells. 
      Asp and Not also suppressed LPS-induced hyperpermeability and leukocyte migration 
      in vivo. Furthermore, each compound suppressed the production of tumor necrosis 
      factor-alpha (TNF-alpha) or interleukin (IL)-6 and the activation of nuclear factor-kappaB 
      (NF-kappaB) or extracellular regulated kinases (ERK) 1/2 by LPS. Moreover, treatment 
      with each compound resulted in reduced LPS-induced lethal endotoxemia. These 
      results suggest that Asp and Not posses anti-inflammatory functions by inhibiting 
      hyperpermeability, expression of CAMs, and adhesion and migration of leukocytes, 
      thereby endorsing its usefulness as a therapy for vascular inflammatory diseases.
FAU - Lee, Wonhwa
AU  - Lee W
AD  - College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, 
      Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu, 702-701, Republic of 
      Korea.
FAU - Bae, Jong-Sup
AU  - Bae JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chalcones)
RN  - 0 (Inflammation Mediators)
RN  - 0 (aspalathin)
RN  - 0 (nothofagin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Movement/drug effects/physiology
MH  - Chalcones/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Human Umbilical Vein Endothelial Cells/drug effects/metabolism
MH  - Humans
MH  - Inflammation Mediators/*antagonists & inhibitors/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neutrophils/drug effects/metabolism
EDAT- 2015/02/07 06:00
MHDA- 2016/04/19 06:00
CRDT- 2015/02/07 06:00
PHST- 2015/02/07 06:00 [entrez]
PHST- 2015/02/07 06:00 [pubmed]
PHST- 2016/04/19 06:00 [medline]
AID - 10.1007/s10753-015-0125-1 [doi]
PST - ppublish
SO  - Inflammation. 2015 Aug;38(4):1502-16. doi: 10.1007/s10753-015-0125-1.