PMID- 2565621
OWN - NLM
STAT- MEDLINE
DCOM- 19890602
LR  - 20190919
IS  - 0340-6075 (Print)
IS  - 0340-6075 (Linking)
VI  - 56
IP  - 4
DP  - 1989
TI  - Evaluation of lysosomal stability in living cultured macrophages by 
      cytofluorometry. Effect of silver lactate and hypotonic conditions.
PG  - 263-9
AB  - The ability of living mouse peritoneal macrophages to retain the lysosomotropic 
      photosensitizer acridine orange (AO) within their secondary lysosomes was studied 
      with a novel cytofluorometric method. During exposure to blue light, cellular AO 
      fluorescence turned from a red granular pattern to that of diffuse green. The 
      resulting change in total fluorescence intensity versus time - a primary decline 
      due to red fluorescence bleaching and a secondary recovery due to the spectral 
      shift - was interpreted as the result of leakage of AO from the lysosomal 
      vacuome. The hypothesis that this time course should be affected by changes in 
      lysosomal membrane stability was tested by labilizing the lysosomes by exposure 
      of cultured macrophages to either hypotonic medium or silver lactate. In 
      hypotonic medium, the ability to retain AO decreased continuously. Exposure to 
      low concentrations of silver lactate (10 microM) also decreased AO retention 
      time. We suggest that this method could be used, within appropriate experimental 
      conditions, to evaluate lysosomal membrane stability in living cells.
FAU - Olsson, G M
AU  - Olsson GM
AD  - Department of Pathology, Linkoping University, Sweden.
FAU - Rungby, J
AU  - Rungby J
FAU - Rundquist, I
AU  - Rundquist I
FAU - Brunk, U T
AU  - Brunk UT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Virchows Arch B Cell Pathol Incl Mol Pathol
JT  - Virchows Archiv. B, Cell pathology including molecular pathology
JID - 9316922
RN  - 0 (Culture Media)
RN  - 0 (Lactates)
RN  - 33X04XA5AT (Lactic Acid)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cells, Cultured
MH  - Culture Media
MH  - Flow Cytometry
MH  - In Vitro Techniques
MH  - Lactates
MH  - Lactic Acid
MH  - Light/adverse effects
MH  - Lysosomes/*analysis/drug effects
MH  - Macrophages/drug effects/*pathology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Spectrometry, Fluorescence
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1007/BF02890025 [doi]
PST - ppublish
SO  - Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;56(4):263-9. doi: 
      10.1007/BF02890025.