PMID- 25656491
OWN - NLM
STAT- MEDLINE
DCOM- 20160122
LR  - 20171116
IS  - 2192-2659 (Electronic)
IS  - 2192-2640 (Linking)
VI  - 4
IP  - 6
DP  - 2015 Apr 22
TI  - Nanostructured Architectures by Assembling Polysaccharide-Coated BSA 
      Nanoparticles for Biomedical Application.
PG  - 927-37
LID - 10.1002/adhm.201400684 [doi]
AB  - Nanostructured architectures are produced on Ti surfaces by layer-by layer (LbL) 
      self-assembling of polysaccharide-coated BSA nanoparticles (BNPs), which created 
      cellular microenvironments mimicking natural extracellular matrix. The BMP-2 
      encapsulated BNPs are prepared by a desolvation method, and are further coated by 
      chitosan (CHI) coatings to obtain positively charged NPs (CBNPs). Vancomycin 
      (Van) encapsulated CBNPs are obtained by the same method and subsequently coated 
      by oxidized alginate (OALG) to obtain negatively charged NPs (OCBNPs). The CBNPs 
      and OCBNPs are assembled on Ti surfaces to construct nanostructured coatings via 
      electrostatic and covalent interactions. The nanostructured architectures realize 
      the sustained release of BMP-2 and Van for a long term. Bone marrow stromal cells 
      (BMSCs) culture tests confirm that the bare nanostructured architectures 
      intrinsically facilitate attachment, proliferation, and differentiation of cells, 
      which is attributed to the nanoscale porous structures that are similar to the 
      size of cellular filopodia. Incorporating BMP-2 into the nanostructured 
      architectures significantly enhances osteogenetic differentiation of BMSCs, which 
      reveals the synergistic effects of nanostructures and growth factors on cell 
      activity. The antibacterial tests indicate that controlled release of Van has 
      good antibacterial ability against Staphylococcus epidermidis, while not 
      affecting the normal biological activity of BMSCs.
CI  - (c) 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Wang, Zhenming
AU  - Wang Z
AD  - School of Materials Science and Engineering, Southwest Jiaotong University, 
      Chengdu, 610031, Sichuan, China.
FAU - Wang, Kefeng
AU  - Wang K
FAU - Lu, Xiong
AU  - Lu X
FAU - Li, Chen
AU  - Li C
FAU - Han, Lu
AU  - Han L
FAU - Xie, Chaoming
AU  - Xie C
FAU - Liu, Yaling
AU  - Liu Y
FAU - Qu, Shuxin
AU  - Qu S
FAU - Zhen, Guanming
AU  - Zhen G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150205
PL  - Germany
TA  - Adv Healthc Mater
JT  - Advanced healthcare materials
JID - 101581613
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Polysaccharides)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Anti-Bacterial Agents/administration & dosage
MH  - Cells, Cultured
MH  - *Coated Materials, Biocompatible
MH  - Humans
MH  - *Nanostructures
MH  - Polysaccharides/*chemistry
MH  - Serum Albumin, Bovine/*chemistry
OTO - NOTNLM
OT  - bovine serum albumin
OT  - dual delivery
OT  - nanoparticles
OT  - nanostructured architectures
OT  - vancomycin
EDAT- 2015/02/07 06:00
MHDA- 2016/01/23 06:00
CRDT- 2015/02/07 06:00
PHST- 2014/11/04 00:00 [received]
PHST- 2015/01/08 00:00 [revised]
PHST- 2015/02/07 06:00 [entrez]
PHST- 2015/02/07 06:00 [pubmed]
PHST- 2016/01/23 06:00 [medline]
AID - 10.1002/adhm.201400684 [doi]
PST - ppublish
SO  - Adv Healthc Mater. 2015 Apr 22;4(6):927-37. doi: 10.1002/adhm.201400684. Epub 
      2015 Feb 5.