PMID- 25656649
OWN - NLM
STAT- MEDLINE
DCOM- 20150715
LR  - 20181113
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 230
IP  - 8
DP  - 2015 Aug
TI  - MicroRNA-214 Is Upregulated in Heart Failure Patients and Suppresses 
      XBP1-Mediated Endothelial Cells Angiogenesis.
PG  - 1964-73
LID - 10.1002/jcp.24942 [doi]
AB  - More and more miRNAs have been shown to regulate gene expression in the heart and 
      dysregulation of their expression has been linked to cardiovascular diseases 
      including the miR-199a/214 cluster. However, the signature of circulating miR-214 
      expression and its possible roles during the development of heart failure has 
      been less well studied. In this study, we elucidated the biological and clinical 
      significance of miR-214 dysregulation in heart failure. Firstly, circulating 
      miR-214 was measured by quantitative PCR, and we found that miR-214 was 
      upregulated in the serum of chronic heart failure patients, as well as in 
      hypertrophic and failing hearts of humans and mice. Adeno-associated virus 
      serotype 9 (AAV9)-mediated miR-214 silencing attenuates isoproterenol (ISO) 
      infusion-induced cardiac dysfunction and impairment of cardiac angiogenesis in 
      mice. Mechanistically, miR-214 overexpression reduces angiogenesis of HUVECs by 
      targeting XBP1, an important transcription factor of unfolded protein response, 
      and XBP1 silencing decreases HUVECs proliferation and angiogenesis similar to 
      miR-214 overexpression. Furthermore, ectopic expression of XBP1 enhances 
      endothelial cells proliferation and tube formation, and reverses anti-angiogenic 
      effect of miR-214 over expression. All these findings suggest that miR-214 is an 
      important regulator of angiogenesis in heart in vitro and in vivo, likely via 
      regulating the expression of XBP1, and demonstrate that miR-214 plays an 
      essential role in the control/inhibition of cardiac angiogenesis.
CI  - (c) 2015 The Authors. Journal of Cellular Physiology published by Wiley 
      Periodicals, Inc.
FAU - Duan, Quanlu
AU  - Duan Q
AD  - Department Internal Medicine and the Institute of Hypertension, Tongji Hospital, 
      Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR 
      China.
FAU - Yang, Lei
AU  - Yang L
FAU - Gong, Wei
AU  - Gong W
FAU - Chaugai, Sandip
AU  - Chaugai S
FAU - Wang, Feng
AU  - Wang F
FAU - Chen, Chen
AU  - Chen C
FAU - Wang, Peihua
AU  - Wang P
FAU - Zou, Ming-Hui
AU  - Zou MH
FAU - Wang, Dao Wen
AU  - Wang DW
LA  - eng
GR  - R01 HL089920/HL/NHLBI NIH HHS/United States
GR  - R01 HL128014/HL/NHLBI NIH HHS/United States
GR  - R01 HL074399/HL/NHLBI NIH HHS/United States
GR  - R01 AG047776/AG/NIA NIH HHS/United States
GR  - R01 HL110488/HL/NHLBI NIH HHS/United States
GR  - R01 HL105157/HL/NHLBI NIH HHS/United States
GR  - R01 HL079584/HL/NHLBI NIH HHS/United States
GR  - R01 HL080499/HL/NHLBI NIH HHS/United States
GR  - R01 HL096032/HL/NHLBI NIH HHS/United States
GR  - R01 HL132500/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MIRN214 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Regulatory Factor X Transcription Factors)
RN  - 0 (Transcription Factors)
RN  - 0 (X-Box Binding Protein 1)
RN  - 0 (XBP1 protein, human)
RN  - 0 (Xbp1 protein, mouse)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Blotting, Western
MH  - DNA-Binding Proteins/*metabolism
MH  - Endothelial Cells/*metabolism
MH  - Female
MH  - Gene Expression Regulation/physiology
MH  - Gene Knockdown Techniques
MH  - Heart Failure/*genetics/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - Neovascularization, Physiologic/*genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Regulatory Factor X Transcription Factors
MH  - Transcription Factors/*metabolism
MH  - Up-Regulation
MH  - X-Box Binding Protein 1
PMC - PMC4911176
MID - NIHMS790394
EDAT- 2015/02/07 06:00
MHDA- 2015/07/16 06:00
PMCR- 2016/09/20
CRDT- 2015/02/07 06:00
PHST- 2013/11/21 00:00 [received]
PHST- 2015/01/23 00:00 [accepted]
PHST- 2015/02/07 06:00 [entrez]
PHST- 2015/02/07 06:00 [pubmed]
PHST- 2015/07/16 06:00 [medline]
PHST- 2016/09/20 00:00 [pmc-release]
AID - JCP24942 [pii]
AID - 10.1002/jcp.24942 [doi]
PST - ppublish
SO  - J Cell Physiol. 2015 Aug;230(8):1964-73. doi: 10.1002/jcp.24942.