PMID- 25659113
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20220408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 2
DP  - 2015
TI  - Bursopentin (BP5) protects dendritic cells from lipopolysaccharide-induced 
      oxidative stress for immunosuppression.
PG  - e0117477
LID - 10.1371/journal.pone.0117477 [doi]
LID - e0117477
AB  - Dendritic cells (DCs) play a vital role in the regulation of immune-mediated 
      inflammatory diseases. Thus, DCs have been regarded as a major target for the 
      development of immunomodulators. However, oxidative stress could disturb 
      inflammatory regulation in DCs. Here, we examined the effect of bursopentine 
      (BP5), a novel pentapeptide isolated from chicken bursa of fabricius, on the 
      protection of DCs against oxidative stress for immunosuppression. BP5 showed 
      potent protective effects against the lipopolysaccharide (LPS)-induced oxidative 
      stress in DCs, including nitric oxide, reactive oxygen species and lipid 
      peroxidation. Furthermore, BP5 elevated the level of cellular reductive status 
      through increasing the reduced glutathione (GSH) and the GSH/GSSG ratio. 
      Concomitant with these, the activities of several antioxidative redox enzymes, 
      including glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase 
      (SOD), were obviously enhanced. BP5 also suppressed submucosal DC maturation in 
      the LPS-stimulated intestinal epithelial cells (ECs)/DCs coculture system. 
      Finally, we found that heme oxygenase 1 (HO-1) was remarkably upregulated by BP5 
      in the LPS-induced DCs, and played an important role in the suppression of 
      oxidative stress and DC maturation. These results suggested that BP5 could 
      protect the LPS-activated DCs against oxidative stress and have potential 
      applications in DC-related inflammatory responses.
FAU - Qin, Tao
AU  - Qin T
AD  - Key Lab of Animal Physiology and Biochemistry of China's Department of 
      Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, 
      Nanjing, People's Republic of China.
FAU - Yin, Yinyan
AU  - Yin Y
AD  - Key Lab of Animal Physiology and Biochemistry of China's Department of 
      Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, 
      Nanjing, People's Republic of China.
FAU - Yu, Qinghua
AU  - Yu Q
AD  - Key Lab of Animal Physiology and Biochemistry of China's Department of 
      Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, 
      Nanjing, People's Republic of China.
FAU - Yang, Qian
AU  - Yang Q
AD  - Key Lab of Animal Physiology and Biochemistry of China's Department of 
      Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, 
      Nanjing, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150206
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligopeptides)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (cysteinyl-lysyl-aspartyl-valyl-tyrosine)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Catalase/immunology/metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology/*pathology
MH  - Glutathione/immunology/metabolism
MH  - Glutathione Peroxidase/immunology/metabolism
MH  - Immune Tolerance/*drug effects
MH  - Lipopolysaccharides/*toxicity
MH  - Mice
MH  - Oligopeptides/*pharmacology
MH  - Oxidative Stress/*drug effects/immunology
MH  - Reactive Oxygen Species/immunology/*metabolism
MH  - Superoxide Dismutase/immunology/metabolism
PMC - PMC4319828
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/02/07 06:00
MHDA- 2015/02/07 06:01
PMCR- 2015/02/06
CRDT- 2015/02/07 06:00
PHST- 2014/10/02 00:00 [received]
PHST- 2014/12/23 00:00 [accepted]
PHST- 2015/02/07 06:00 [entrez]
PHST- 2015/02/07 06:00 [pubmed]
PHST- 2015/02/07 06:01 [medline]
PHST- 2015/02/06 00:00 [pmc-release]
AID - PONE-D-14-44369 [pii]
AID - 10.1371/journal.pone.0117477 [doi]
PST - epublish
SO  - PLoS One. 2015 Feb 6;10(2):e0117477. doi: 10.1371/journal.pone.0117477. 
      eCollection 2015.