PMID- 25660453
OWN - NLM
STAT- MEDLINE
DCOM- 20150501
LR  - 20150302
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 458
IP  - 2
DP  - 2015 Mar 6
TI  - The involvement of FAK-PI3K-AKT-Rac1 pathway in porcine reproductive and 
      respiratory syndrome virus entry.
PG  - 392-8
LID - S0006-291X(15)00173-4 [pii]
LID - 10.1016/j.bbrc.2015.01.126 [doi]
AB  - CD163 and sialoadhesin had been reported as the two receptors for porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection. The signaling 
      pathway activated by PRRSV entry was seldom reported. In our studies, we 
      demonstrated that PRRSV entry triggers FAK, PI3K, AKT and Rac1 activation. The 
      signaling pathway FAK-PI3K-AKT-Rac1 is essential for PRRSV entry. Blocking FAK by 
      PF573228 attenuates the activation of PI3K, AKT, Rac1 and the cytoskeleton 
      remodeling induced by virus entry. Inhibitors to FAK, PI3K, AKT and Rac1 can 
      significantly inhibit the virus entry. In conclusion, our observations reveal 
      that PRRSV triggers the activation of FAK-PI3K-AKT-Rac1 signaling pathway to 
      facilitate its entry into cells.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Ni, Bo
AU  - Ni B
AD  - College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, 
      Nanjing, 210095, China.
FAU - Wen, Li-Bin
AU  - Wen LB
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Science, Key 
      Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, National Center for Engineering Research of Veterinary Bio-products, 
      Nanjing, 210014, China.
FAU - Wang, Rui
AU  - Wang R
AD  - College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, 
      Nanjing, 210095, China.
FAU - Hao, Hong-Ping
AU  - Hao HP
AD  - College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, 
      Nanjing, 210095, China.
FAU - Huan, Chang-Chao
AU  - Huan CC
AD  - College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, 
      Nanjing, 210095, China.
FAU - Wang, Xin
AU  - Wang X
AD  - College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, 
      Nanjing, 210095, China.
FAU - Huang, Li
AU  - Huang L
AD  - College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, 
      Nanjing, 210095, China.
FAU - Miao, Jin-Feng
AU  - Miao JF
AD  - College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, 
      Nanjing, 210095, China.
FAU - Fan, Hong-Jie
AU  - Fan HJ
AD  - College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, 
      Nanjing, 210095, China; Jiangsu Co-innovation Center for Prevention and Control 
      of Important Animal, Infectious Diseases and Zoonoses, Yangzhou, 225009, China. 
      Electronic address: fhj@njau.edu.cn.
FAU - Mao, Xiang
AU  - Mao X
AD  - College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, 
      Nanjing, 210095, China. Electronic address: xmao@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150207
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 1)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - Focal Adhesion Kinase 1/metabolism
MH  - Oncogene Protein v-akt/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphotransferases/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*enzymology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Signal Transduction
MH  - Swine/*metabolism/*virology
MH  - *Virus Internalization
MH  - rac1 GTP-Binding Protein/metabolism
OTO - NOTNLM
OT  - FAK
OT  - Infection
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Rac1
EDAT- 2015/02/11 06:00
MHDA- 2015/05/02 06:00
CRDT- 2015/02/10 06:00
PHST- 2015/01/01 00:00 [received]
PHST- 2015/01/26 00:00 [accepted]
PHST- 2015/02/10 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2015/05/02 06:00 [medline]
AID - S0006-291X(15)00173-4 [pii]
AID - 10.1016/j.bbrc.2015.01.126 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Mar 6;458(2):392-8. doi: 
      10.1016/j.bbrc.2015.01.126. Epub 2015 Feb 7.