PMID- 25660474
OWN - NLM
STAT- MEDLINE
DCOM- 20160104
LR  - 20181113
IS  - 1095-9319 (Electronic)
IS  - 0026-2862 (Print)
IS  - 0026-2862 (Linking)
VI  - 98
DP  - 2015 Mar
TI  - Effect of osmolality on erythrocyte rheology and perfusion of an artificial 
      microvascular network.
PG  - 102-7
LID - S0026-2862(15)00011-4 [pii]
LID - 10.1016/j.mvr.2015.01.010 [doi]
AB  - Plasma sodium concentration is normally held within a narrow range. It may 
      however vary greatly under pathophysiological conditions. Changes in osmolality 
      lead to either swelling or shrinkage of red blood cells (RBCs). Here we 
      investigated the influence of suspension osmolality on biophysical properties of 
      RBCs and their ability to perfuse an artificial microvascular network (AMVN). 
      Blood was drawn from healthy volunteers. RBC deformability was measured by 
      osmotic gradient ektacytometry over a continuous range of osmolalities. Packed 
      RBCs were suspended in NaCl solutions (0.45, 0.6, 0.9, 1.2, and 1.5 g/dL), 
      resulting in supernatant osmolalities of 179 +/- 4, 213 +/- 1, 283 +/- 2, 354 +/- 3, and 
      423 +/- 5 mOsm/kg H2O. Mean corpuscular volume (MCV) and mean corpuscular 
      hemoglobin concentration (MCHC) were determined using centrifuged 
      microhematocrit. RBC suspensions at constant cell numbers were used to measure 
      viscosity at shear rates ranging from 0.11 to 69.5s(-1) and the perfusion rate of 
      the AMVN. MCV was inversely and MCHC directly proportional to osmolality. RBC 
      deformability was maximized at isosmotic conditions (290 mOsm/kg H2O) and 
      markedly decreased by either hypo- or hyperosmolality. The optimum osmolality for 
      RBC suspension viscosity was shifted toward hyperosmolality, while lower 
      osmolalities increased suspension viscosity exponentially. However, the AMVN 
      perfusion rate was maximized at 290 mOsm/kg H2O and changed by less than 10% over 
      a wide range of osmolalities. These findings contribute to the basic 
      understanding of blood flow in health and disease and may have significant 
      implications for the management of osmotic homeostasis in clinical practice.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Reinhart, Walter H
AU  - Reinhart WH
AD  - Department of Internal Medicine, Kantonsspital Graubunden, Chur, Switzerland.
FAU - Piety, Nathaniel Z
AU  - Piety NZ
AD  - Department of Biomedical Engineering, Cullen College of Engineering, University 
      of Houston, Houston, TX, USA.
FAU - Goede, Jeroen S
AU  - Goede JS
AD  - Division of Hematology, University Hospital, Zurich, Switzerland.
FAU - Shevkoplyas, Sergey S
AU  - Shevkoplyas SS
AD  - Department of Biomedical Engineering, Cullen College of Engineering, University 
      of Houston, Houston, TX, USA. Electronic address: sshevkoplyas@uh.edu.
LA  - eng
GR  - R01 HL117329/HL/NHLBI NIH HHS/United States
GR  - R01HL117329/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150207
PL  - United States
TA  - Microvasc Res
JT  - Microvascular research
JID - 0165035
RN  - 0 (Hemoglobins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blood Viscosity
MH  - Erythrocyte Count
MH  - Erythrocyte Deformability/physiology
MH  - Erythrocyte Indices
MH  - Erythrocytes/*cytology
MH  - Healthy Volunteers
MH  - Hemoglobins/*chemistry
MH  - Homeostasis
MH  - Humans
MH  - Microcirculation
MH  - Microfluidics
MH  - *Microvessels
MH  - Middle Aged
MH  - Osmolar Concentration
MH  - Perfusion
MH  - *Rheology
MH  - Viscosity
MH  - Young Adult
PMC - PMC4361376
MID - NIHMS661660
OTO - NOTNLM
OT  - Artificial microvascular network
OT  - Hyperosmolality
OT  - Hypo-osmolality
OT  - Microfluidics
OT  - Microvascular perfusion
OT  - Osmolality
OT  - Red blood cell deformability
EDAT- 2015/02/11 06:00
MHDA- 2016/01/05 06:00
PMCR- 2016/03/01
CRDT- 2015/02/10 06:00
PHST- 2015/01/01 00:00 [received]
PHST- 2015/01/27 00:00 [revised]
PHST- 2015/01/29 00:00 [accepted]
PHST- 2015/02/10 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2016/01/05 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - S0026-2862(15)00011-4 [pii]
AID - 10.1016/j.mvr.2015.01.010 [doi]
PST - ppublish
SO  - Microvasc Res. 2015 Mar;98:102-7. doi: 10.1016/j.mvr.2015.01.010. Epub 2015 Feb 
      7.