PMID- 25660973
OWN - NLM
STAT- MEDLINE
DCOM- 20160321
LR  - 20150613
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 79
IP  - 6
DP  - 2015
TI  - Knockdown of the host cellular protein transportin 3 attenuates prototype foamy 
      virus infection.
PG  - 943-51
LID - 10.1080/09168451.2015.1008973 [doi]
AB  - Transportin 3 (TNPO3) is a member of the importin-ss superfamily proteins. Despite 
      numerous studies, the exact molecular mechanism of TNPO3 in retroviral infection 
      is still controversial. Here, we provide evidence for the role and mechanism of 
      TNPO3 in the replication of prototype foamy virus (PFV). Our findings revealed 
      that PFV infection was reduced 2-fold by knockdown (KD) of TNPO3. However, late 
      stage of viral replication including transcription, translation, viral assembly, 
      and release was not influenced. The differential cellular localization of PFV 
      integrase (IN) in KD cells pinpointed a remarkable reduction of viral replication 
      at the nuclear import step. We also found that TNPO3 interacted with PFV IN but 
      not with Gag, suggesting that IN-TNPO3 interaction is important for nuclear 
      import of PFV pre-integration complex. Our report enlightens the mechanism of PFV 
      interaction with TNPO3 and support ongoing research on PFV as a promising safe 
      vector for gene therapy.
FAU - Ali, Md Khadem
AU  - Ali MK
AD  - a Department of Systems Biotechnology , Chung Ang University , Ansung , Republic 
      of Korea.
FAU - Kim, Jinsun
AU  - Kim J
FAU - Hamid, Faysal Bin
AU  - Hamid FB
FAU - Shin, Cha-Gyun
AU  - Shin CG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150209
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (beta Karyopherins)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cricetinae
MH  - *Gene Knockdown Techniques
MH  - Integrases/metabolism
MH  - Spumavirus/enzymology/*physiology
MH  - beta Karyopherins/*deficiency/*genetics
OTO - NOTNLM
OT  - integrase
OT  - pre-integration complex
OT  - prototype foamy virus
OT  - transportin 3
EDAT- 2015/02/11 06:00
MHDA- 2016/03/22 06:00
CRDT- 2015/02/10 06:00
PHST- 2015/02/10 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2016/03/22 06:00 [medline]
AID - 10.1080/09168451.2015.1008973 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2015;79(6):943-51. doi: 10.1080/09168451.2015.1008973. 
      Epub 2015 Feb 9.