PMID- 25661728
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 2042-0226 (Electronic)
IS  - 1672-7681 (Print)
IS  - 1672-7681 (Linking)
VI  - 13
IP  - 2
DP  - 2016 Mar
TI  - Phenotypic and functional comparison of two distinct subsets of programmable cell 
      of monocytic origin (PCMOs)-derived dendritic cells with conventional 
      monocyte-derived dendritic cells.
PG  - 160-9
LID - 10.1038/cmi.2014.135 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells with the ability 
      to induce primary T-cell responses. They are commonly produced by culturing 
      monocytes in the presence of IL-4 and GM-CSF (cells produced in this manner are 
      called conventional DCs). Here we report the generation of two functionally 
      distinct subsets of DCs derived from programmable cells of monocytic origin 
      (PCMOs) in the presence of IL-3 or tumor necrosis factor alpha (TNF-alpha). Monocytes 
      were treated with macrophage colony-stimulating factor (M-CSF) and IL-3 for 6 
      days and then incubated with IL-4 and IL-3 (for IL-3 DCs) or with IL-4, GM-CSF 
      and TNF-alpha (for TNF-alpha DCs) for 7 days. Monocytes were then loaded with tumor 
      lysate (used as antigen), and poly (IratioC) was added. The maturation factors TNF-alpha 
      and monocyte conditioned medium (MCM) were added on days 4 and 5, respectively. 
      The phenotypes of the DCs generated were characterized by flow cytometry, and the 
      cells' phagocytic activities were measured using FITC-conjugated latex bead 
      uptake. T-cell proliferation and cytokine release were assayed using MTT and 
      commercially available ELISA kits, respectively. We found that either IL-3DCs or 
      TNF-alpha DCs induce T-cell proliferation and cytokine secretion; the cytokine 
      release pattern showed reduced IL-12/IL-10 and IFN-gamma/IL-4 ratios in both types of 
      DCs and in DC-primed T-cell supernatant, respectively, which confirmed that the 
      primed T cells were polarized toward aTh2-type immune response. We concluded that 
      PCMOs are a new cell source that can develop into two functionally distinct DCs 
      that both induce a Th2-type response in vitro. This modality can be used as a 
      DC-based immunotherapy for autoimmune diseases.
FAU - Beikzadeh, Babak
AU  - Beikzadeh B
AD  - Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, 
      Urmia, Iran.
FAU - Delirezh, Nowruz
AU  - Delirezh N
AD  - Department of Cellular and Molecular Biotechnology, Institute of Biotechnology, 
      Urmia University, Urmia, Iran.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150209
PL  - China
TA  - Cell Mol Immunol
JT  - Cellular & molecular immunology
JID - 101242872
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cytokines)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Antigens, Neoplasm/immunology
MH  - Breast Neoplasms/*immunology/pathology
MH  - Coculture Techniques
MH  - Cytokines/*immunology
MH  - Dendritic Cells/*immunology/pathology
MH  - Female
MH  - Humans
MH  - Monocytes/*immunology/pathology
MH  - Poly I-C/pharmacology
MH  - Th2 Cells/immunology/pathology
PMC - PMC4786623
EDAT- 2015/02/11 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/03/01
CRDT- 2015/02/10 06:00
PHST- 2014/07/01 00:00 [received]
PHST- 2014/12/18 00:00 [revised]
PHST- 2014/12/18 00:00 [accepted]
PHST- 2015/02/10 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - cmi2014135 [pii]
AID - 10.1038/cmi.2014.135 [doi]
PST - ppublish
SO  - Cell Mol Immunol. 2016 Mar;13(2):160-9. doi: 10.1038/cmi.2014.135. Epub 2015 Feb 
      9.