PMID- 25662274
OWN - NLM
STAT- MEDLINE
DCOM- 20160208
LR  - 20211203
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 408
DP  - 2015 Jun 15
TI  - Stimulation of StAR expression by cAMP is controlled by inhibition of highly 
      inducible SIK1 via CRTC2, a co-activator of CREB.
PG  - 80-9
LID - S0303-7207(15)00026-X [pii]
LID - 10.1016/j.mce.2015.01.022 [doi]
AB  - In mouse steroidogenic cells the activation of cholesterol metabolism is mediated 
      by steroidogenic acute regulatory protein (StAR). Here, we visualized a 
      coordinated regulation of StAR transcription, splicing and post-transcriptional 
      processing, which are synchronized by salt inducible kinase (SIK1) and 
      CREB-regulated transcription coactivator (CRTC2). To detect primary RNA (pRNA), 
      spliced primary RNA (Sp-RNA) and mRNA in single cells, we generated probe sets by 
      using fluorescence in situ hybridization (FISH). These methods allowed us to 
      address the nature of StAR gene expression and to visualize protein-nucleic acid 
      interactions through direct detection. We show that SIK1 represses StAR 
      expression in Y1 adrenal and MA10 testis cells through inhibition of processing 
      mediated by CRTC2. Digital image analysis matches qPCR analyses of the total cell 
      culture. Evidence is presented for spatially separate accumulation of StAR pRNA 
      and Sp-RNA at the gene loci in the nucleus. These findings establish that cAMP, 
      SIK and CRTC mediate StAR expression through activation of individual StAR gene 
      loci.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Lee, Jinwoo
AU  - Lee J
AD  - Department of Cell and Regenerative Biology, University of Wisconsin, Madison, 
      WI, USA; Endocrinology and Reproductive Physiology Program, University of 
      Wisconsin, Madison, WI, USA.
FAU - Tong, Tiegang
AU  - Tong T
AD  - Department of Cell and Regenerative Biology, University of Wisconsin, Madison, 
      WI, USA.
FAU - Takemori, Hiroshi
AU  - Takemori H
AD  - National Institute for Biomedical Innovation, Osaka, Japan.
FAU - Jefcoate, Colin
AU  - Jefcoate C
AD  - Department of Cell and Regenerative Biology, University of Wisconsin, Madison, 
      WI, USA; Endocrinology and Reproductive Physiology Program, University of 
      Wisconsin, Madison, WI, USA; University of Wisconsin School of Medicine and 
      Public Health, Madison, WI, USA. Electronic address: jefcoate@wisc.edu.
LA  - eng
GR  - R01 DK074819/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150207
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Crtc2 protein, mouse)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Sik1 protein, mouse)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - Alternative Splicing/drug effects/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/drug effects/metabolism
MH  - Cyclic AMP/*pharmacology
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Enzyme Induction/drug effects
MH  - Green Fluorescent Proteins/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Phosphoproteins/*genetics/metabolism
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Protein Transport/drug effects
MH  - RNA Precursors/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Transcription Factors/chemistry/*metabolism
MH  - Transcription, Genetic/drug effects
PMC - PMC4417451
MID - NIHMS667353
OTO - NOTNLM
OT  - CRTC
OT  - Fluorescence in situ hybridization
OT  - SIK
OT  - Splicing
OT  - StAR
EDAT- 2015/02/11 06:00
MHDA- 2016/02/09 06:00
PMCR- 2016/06/15
CRDT- 2015/02/10 06:00
PHST- 2014/10/13 00:00 [received]
PHST- 2015/01/15 00:00 [revised]
PHST- 2015/01/15 00:00 [accepted]
PHST- 2015/02/10 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2016/02/09 06:00 [medline]
PHST- 2016/06/15 00:00 [pmc-release]
AID - S0303-7207(15)00026-X [pii]
AID - 10.1016/j.mce.2015.01.022 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2015 Jun 15;408:80-9. doi: 10.1016/j.mce.2015.01.022. Epub 
      2015 Feb 7.