PMID- 25662275
OWN - NLM
STAT- MEDLINE
DCOM- 20151221
LR  - 20150317
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 405
DP  - 2015 Apr 15
TI  - PRDM16 sustains white fat gene expression profile in human adipocytes in direct 
      relation with insulin action.
PG  - 84-93
LID - S0303-7207(15)00066-0 [pii]
LID - 10.1016/j.mce.2015.01.042 [doi]
AB  - In the present study, we aimed to evaluate the possible role of PRDM16 in human 
      adipocytes and in whole adipose tissue according to obesity and insulin 
      sensitivity. PRDM16 knockdown (KD) had a dual behavior. While KD in preadipocytes 
      led to enhanced gene expression markers of adipocyte differentiation, PRDM16 KD 
      in fully differentiated adipocytes resulted in decreased adipogenic gene 
      expression and insulin action. In line with KD in adipocytes, PRDM16 was 
      positively associated with the expression of several genes involved in 
      adipogenesis, insulin signaling, mitochondrial function and brown 
      adipocyte-related markers in whole adipose tissue from two independent cohorts. 
      PRDM16 was decreased in obese subjects in relation with the decrease of insulin 
      sensitivity [HOM(AIR) (cohort 1) and M clamp value (cohort 2)]. Rosiglitazone (5 
      micromol/l) and metformin (5 mmol/l) led to increased PRDM16 mRNA and protein levels 
      in isolated human adipocytes and in whole adipose tissue. In conclusion, PRDM16 
      might contribute to maintain adipose tissue "white fat" gene expression profile 
      and systemic metabolic homeostasis.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Moreno-Navarrete, Jose Maria
AU  - Moreno-Navarrete JM
AD  - Department of Diabetes, Endocrinology and Nutrition, Institut d'Investigacio 
      Biomedica de Girona (IdIBGi), CIBEROBN (CB06/03/010), Instituto de Salud Carlos 
      III (ISCIII), Girona 17007, Spain. Electronic address: jmoreno@idibgi.org.
FAU - Ortega, Francisco
AU  - Ortega F
AD  - Department of Diabetes, Endocrinology and Nutrition, Institut d'Investigacio 
      Biomedica de Girona (IdIBGi), CIBEROBN (CB06/03/010), Instituto de Salud Carlos 
      III (ISCIII), Girona 17007, Spain.
FAU - Moreno, Maria
AU  - Moreno M
AD  - Department of Diabetes, Endocrinology and Nutrition, Institut d'Investigacio 
      Biomedica de Girona (IdIBGi), CIBEROBN (CB06/03/010), Instituto de Salud Carlos 
      III (ISCIII), Girona 17007, Spain.
FAU - Xifra, Gemma
AU  - Xifra G
AD  - Department of Diabetes, Endocrinology and Nutrition, Institut d'Investigacio 
      Biomedica de Girona (IdIBGi), CIBEROBN (CB06/03/010), Instituto de Salud Carlos 
      III (ISCIII), Girona 17007, Spain.
FAU - Ricart, Wifredo
AU  - Ricart W
AD  - Department of Diabetes, Endocrinology and Nutrition, Institut d'Investigacio 
      Biomedica de Girona (IdIBGi), CIBEROBN (CB06/03/010), Instituto de Salud Carlos 
      III (ISCIII), Girona 17007, Spain.
FAU - Fernandez-Real, Jose Manuel
AU  - Fernandez-Real JM
AD  - Department of Diabetes, Endocrinology and Nutrition, Institut d'Investigacio 
      Biomedica de Girona (IdIBGi), CIBEROBN (CB06/03/010), Instituto de Salud Carlos 
      III (ISCIII), Girona 17007, Spain. Electronic address: jmfreal@idibgi.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150207
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (PRDM16 protein, human)
RN  - 0 (SLC2A4 protein, human)
RN  - 0 (Transcription Factors)
RN  - 9100L32L2N (Metformin)
SB  - IM
MH  - Adipocytes, White/*metabolism
MH  - Adipogenesis
MH  - Adipose Tissue, White/metabolism/pathology
MH  - Adult
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/*physiology
MH  - Diabetes Mellitus, Type 2/metabolism
MH  - Female
MH  - Glucose Transporter Type 4/metabolism
MH  - Homeostasis
MH  - Humans
MH  - Hypoglycemic Agents/pharmacology
MH  - Insulin/*physiology
MH  - Insulin Resistance
MH  - Male
MH  - Metformin/pharmacology
MH  - Middle Aged
MH  - Obesity/metabolism
MH  - Transcription Factors/*physiology
MH  - *Transcriptome
OTO - NOTNLM
OT  - Adipogenesis
OT  - Human adipose tissue
OT  - Insulin sensitivity
OT  - Obesity
OT  - PRDM16
EDAT- 2015/02/11 06:00
MHDA- 2015/12/22 06:00
CRDT- 2015/02/10 06:00
PHST- 2014/10/13 00:00 [received]
PHST- 2015/01/22 00:00 [revised]
PHST- 2015/01/29 00:00 [accepted]
PHST- 2015/02/10 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2015/12/22 06:00 [medline]
AID - S0303-7207(15)00066-0 [pii]
AID - 10.1016/j.mce.2015.01.042 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2015 Apr 15;405:84-93. doi: 10.1016/j.mce.2015.01.042. Epub 
      2015 Feb 7.