PMID- 25662311
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20180429
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 477
DP  - 2015 Mar
TI  - Functional analysis of porcine reproductive and respiratory syndrome virus 
      N-glycans in infection of permissive cells.
PG  - 82-88
LID - S0042-6822(15)00006-9 [pii]
LID - 10.1016/j.virol.2015.01.005 [doi]
AB  - The role of envelope protein-linked N-glycans in porcine reproductive and 
      respiratory syndrome virus (PRRSV) infection of permissive cells was examined. 
      N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomer-specific 
      lectins bound to PRRSV and blocked virus attachment, resulting in reduced viral 
      infection. However, addition of GlcNAc oligomers and LacNAc to cell culture 
      together with PRRSV did not block infection. Removal or alteration of envelope 
      protein-linked N-glycans also did not affect virus infection, indicating that 
      PRRSV N-glycans are not required for virus infection. These findings show that 
      steric hindrance of glycans on the PRRSV envelope by lectins or, presumably, 
      other space-filling molecules, may interfere nonspecifically with infection by 
      blocking protein interactions with cell surface receptors. Glycans themselves 
      appear not to be required for infection of permissive cells, but may have 
      important roles in avoidance of host immunity and in protein structure, 
      intracellular virion growth and assembly.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Li, Juan
AU  - Li J
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 
      Commonwealth Avenue, St. Paul, MN 55108, USA.
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 
      Commonwealth Avenue, St. Paul, MN 55108, USA. Electronic address: 
      murta001@umn.edu.
LA  - eng
GR  - P41 RR018502/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150206
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Lectins)
RN  - 0 (Polysaccharides)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Lectins/metabolism
MH  - Polysaccharides/*metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*chemistry/*physiology
MH  - Swine
MH  - Viral Envelope Proteins/*chemistry/*metabolism
MH  - Virus Attachment/drug effects
OTO - NOTNLM
OT  - Envelope glycoprotein
OT  - GlcNAc oligomer
OT  - LacNAc oligomer
OT  - N-glycan
OT  - PRRSV
OT  - Sialic acid
OT  - Virus infectivity
EDAT- 2015/02/11 06:00
MHDA- 2015/05/15 06:00
CRDT- 2015/02/10 06:00
PHST- 2015/01/03 00:00 [received]
PHST- 2015/01/06 00:00 [accepted]
PHST- 2015/02/10 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
AID - S0042-6822(15)00006-9 [pii]
AID - 10.1016/j.virol.2015.01.005 [doi]
PST - ppublish
SO  - Virology. 2015 Mar;477:82-88. doi: 10.1016/j.virol.2015.01.005. Epub 2015 Feb 6.