PMID- 25662411
OWN - NLM
STAT- MEDLINE
DCOM- 20150424
LR  - 20150303
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 144
IP  - 3
DP  - 2014
TI  - A comprehensive whole-genome integrated cytogenetic map for the alpaca (Lama 
      pacos).
PG  - 196-207
LID - 10.1159/000370329 [doi]
AB  - Genome analysis of the alpaca (Lama pacos, LPA) has progressed slowly compared to 
      other domestic species. Here, we report the development of the first 
      comprehensive whole-genome integrated cytogenetic map for the alpaca using 
      fluorescence in situ hybridization (FISH) and CHORI-246 BAC library clones. The 
      map is comprised of 230 linearly ordered markers distributed among all 36 alpaca 
      autosomes and the sex chromosomes. For the first time, markers were assigned to 
      LPA14, 21, 22, 28, and 36. Additionally, 86 genes from 15 alpaca chromosomes were 
      mapped in the dromedary camel (Camelus dromedarius, CDR), demonstrating 
      exceptional synteny and linkage conservation between the 2 camelid genomes. 
      Cytogenetic mapping of 191 protein-coding genes improved and refined the known 
      Zoo-FISH homologies between camelids and humans: we discovered new homologous 
      synteny blocks (HSBs) corresponding to HSA1-LPA/CDR11, HSA4-LPA/CDR31 and 
      HSA7-LPA/CDR36, and revised the location of breakpoints for others. Overall, gene 
      mapping was in good agreement with the Zoo-FISH and revealed remarkable 
      evolutionary conservation of gene order within many human-camelid HSBs. Most 
      importantly, 91 FISH-mapped markers effectively integrated the alpaca 
      whole-genome sequence and the radiation hybrid maps with physical chromosomes, 
      thus facilitating the improvement of the sequence assembly and the discovery of 
      genes of biological importance.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Avila, Felipe
AU  - Avila F
AD  - Department of Veterinary Integrative Biosciences, College of Veterinary Medicine 
      and Biomedical Sciences, Texas A&M University, College Station, Tex., USA.
FAU - Baily, Malorie P
AU  - Baily MP
FAU - Perelman, Polina
AU  - Perelman P
FAU - Das, Pranab J
AU  - Das PJ
FAU - Pontius, Joan
AU  - Pontius J
FAU - Chowdhary, Renuka
AU  - Chowdhary R
FAU - Owens, Elaine
AU  - Owens E
FAU - Johnson, Warren E
AU  - Johnson WE
FAU - Merriwether, David A
AU  - Merriwether DA
FAU - Raudsepp, Terje
AU  - Raudsepp T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150206
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Animals
MH  - Camelids, New World/*genetics
MH  - Chromosome Mapping
MH  - *Cytogenetic Analysis
MH  - Genetic Linkage
MH  - *Genome
MH  - Humans
MH  - Microsatellite Repeats/genetics
MH  - Synteny
EDAT- 2015/02/11 06:00
MHDA- 2015/04/25 06:00
CRDT- 2015/02/10 06:00
PHST- 2014/10/20 00:00 [accepted]
PHST- 2015/02/10 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2015/04/25 06:00 [medline]
AID - 000370329 [pii]
AID - 10.1159/000370329 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2014;144(3):196-207. doi: 10.1159/000370329. Epub 2015 Feb 
      6.