PMID- 25663024
OWN - NLM
STAT- MEDLINE
DCOM- 20150917
LR  - 20181202
IS  - 1573-7438 (Electronic)
IS  - 0049-4747 (Linking)
VI  - 47
IP  - 3
DP  - 2015 Mar
TI  - Detection of Babesia bigemina infection in cattle from north-eastern India by 
      polymerase chain reaction and its genetic relatedness with other isolates.
PG  - 633-6
LID - 10.1007/s11250-015-0769-8 [doi]
AB  - A total of 333 blood samples were collected from cattle suspected for 
      haemoprotozoan infections from three states of north-eastern part of India. All 
      the samples were examined for diagnosis of Babesia bigemina infection using PCR 
      for detection of specific DNA. Out of these, 12 (3.60%) samples were found 
      positive for B. bigemina DNA on PCR using the organism-specific primers derived 
      from 18S ribosomal RNA (rRNA) gene of B. bigemina. An expected size of 1124-bp 
      PCR product was visualized on agarose gel electrophoresis with all the 12 
      samples, and four of the products was further cloned and sequenced. Basic Local 
      Alignment Search Tool (BLAST) analysis of B. bigemina sequences generated in the 
      present study share 99.2 to 99.7% identity at 18S rRNA gene nucleotide sequence 
      level. These Indian B. bigemina sequences were found to be closely related with 
      the cognate gene nucleotide sequences of B. bigemina from Argentina and Kenya 
      where 99.1 to 99.9% and 99.0 to 99.7% nucleotide identities were observed, 
      respectively. Distant relationship of these Indian organisms was observed with 
      few cognate gene sequences from China where more than 7% divergence was observed 
      in the distance matrix.
FAU - Laha, Ramgopal
AU  - Laha R
AD  - Division of Animal Health, ICAR Research Complex for NEH Region, Umroi Road, 
      Umiam, Meghalaya, 793103, India, lahar31@rediffmail.com.
FAU - Mondal, Bimalendu
AU  - Mondal B
FAU - Biswas, Sanchay Kumar
AU  - Biswas SK
FAU - Chand, Karam
AU  - Chand K
FAU - Das, Meena
AU  - Das M
FAU - Sarma, Diganta
AU  - Sarma D
FAU - Goswami, Anupananda
AU  - Goswami A
FAU - Sen, Arnab
AU  - Sen A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150208
PL  - United States
TA  - Trop Anim Health Prod
JT  - Tropical animal health and production
JID - 1277355
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Protozoan)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Animals
MH  - Babesia/genetics/*isolation & purification
MH  - Babesiosis/*epidemiology/parasitology
MH  - Cattle
MH  - Cattle Diseases/*epidemiology/parasitology
MH  - DNA Primers/analysis
MH  - DNA, Protozoan/genetics
MH  - India/epidemiology
MH  - Phylogeny
MH  - Polymerase Chain Reaction/veterinary
MH  - RNA, Ribosomal, 18S/analysis
EDAT- 2015/02/11 06:00
MHDA- 2015/09/18 06:00
CRDT- 2015/02/10 06:00
PHST- 2014/10/24 00:00 [received]
PHST- 2015/01/12 00:00 [accepted]
PHST- 2015/02/10 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2015/09/18 06:00 [medline]
AID - 10.1007/s11250-015-0769-8 [doi]
PST - ppublish
SO  - Trop Anim Health Prod. 2015 Mar;47(3):633-6. doi: 10.1007/s11250-015-0769-8. Epub 
      2015 Feb 8.