PMID- 2566592
OWN - NLM
STAT- MEDLINE
DCOM- 19890630
LR  - 20190723
IS  - 0021-8820 (Print)
IS  - 0021-8820 (Linking)
VI  - 42
IP  - 5
DP  - 1989 May
TI  - Selective killing of human T cell lymphotropic virus type I-transformed cell 
      lines by a damavaricin Fc derivative.
PG  - 779-87
AB  - n-Pentyl ether of damavaricin Fc (n-pentyl DvFc) preferentially killed human 
      T-cell lymphotropic virus type I (HTLV-I)-transformed cell lines. The mechanism 
      of action of the drug was investigated using MT-4 cells. Cytotoxic action was 
      diminished by the removal of n-pentyl DvFc from the culture or by the addition of 
      sulfhydryl compounds such as 2-mercaptoethanol and dithiothreitol. The killing 
      activity of n-pentyl DvFc was also diminished by membrane-acting agents including 
      quinidine and diphenylhydantoin. Influx and subsequent efflux of Ca2+ were 
      observed when either HTLV-I infected (MT-4 cells) or uninfected cells were 
      treated with n-pentyl DvFc. An efflux of K+ was observed in HTLV-I infected MT-4 
      cells immediately after the exposure of the cells to n-pentyl DvFc. The K+ 
      efflux, however, was not observed in the uninfected T cells. n-Pentyl DvFc seems 
      to act primarily on the cell surface of MT-4 cells, leading to the perturbation 
      of membrane function. The restoration of cell growth, however, is critically 
      dependent on the presence of dithiothreitol and 2-mercaptoethanol, implying a 
      role for a free sulfhydryl group in the killing activity.
FAU - Ito, S
AU  - Ito S
AD  - Shin-Etsu Chemical Co., Ltd., Tokyo.
FAU - Yamamoto, N
AU  - Yamamoto N
FAU - Nomoto, K
AU  - Nomoto K
FAU - Sasaki, K
AU  - Sasaki K
FAU - Onodera, K
AU  - Onodera K
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Antibiot (Tokyo)
JT  - The Journal of antibiotics
JID - 0151115
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antiviral Agents)
RN  - 1404-74-6 (Streptovaricin)
RN  - 60-24-2 (Mercaptoethanol)
RN  - 6158TKW0C5 (Phenytoin)
RN  - 70940-04-4 (damavaricin Fc pentyl ether)
RN  - 9007-49-2 (DNA)
RN  - ITX08688JL (Quinidine)
RN  - RWP5GA015D (Potassium)
RN  - SY7Q814VUP (Calcium)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Anti-Bacterial Agents
MH  - Antiviral Agents/*pharmacology/therapeutic use
MH  - Calcium/metabolism
MH  - Cell Line, Transformed
MH  - Cell Membrane/drug effects/metabolism
MH  - Cell Membrane Permeability/drug effects
MH  - Cell Survival/drug effects
MH  - Cell Transformation, Neoplastic
MH  - Cell Transformation, Viral
MH  - DNA/biosynthesis
MH  - Dithiothreitol/pharmacology
MH  - *Human T-lymphotropic virus 1
MH  - Humans
MH  - Leukemia, T-Cell/drug therapy/pathology
MH  - Mercaptoethanol/pharmacology
MH  - Phenytoin/pharmacology
MH  - Potassium/metabolism
MH  - Quinidine/pharmacology
MH  - Streptovaricin/*pharmacology/therapeutic use
MH  - T-Lymphocytes/*drug effects/metabolism/microbiology
EDAT- 1989/05/01 00:00
MHDA- 1989/05/01 00:01
CRDT- 1989/05/01 00:00
PHST- 1989/05/01 00:00 [pubmed]
PHST- 1989/05/01 00:01 [medline]
PHST- 1989/05/01 00:00 [entrez]
AID - 10.7164/antibiotics.42.779 [doi]
PST - ppublish
SO  - J Antibiot (Tokyo). 1989 May;42(5):779-87. doi: 10.7164/antibiotics.42.779.