PMID- 25666453
OWN - NLM
STAT- MEDLINE
DCOM- 20160502
LR  - 20191210
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 176
IP  - 3-4
DP  - 2015 Apr 17
TI  - Virological and serological investigation of Equid herpesvirus 1 infection in New 
      Zealand.
PG  - 219-28
LID - S0378-1135(15)00033-4 [pii]
LID - 10.1016/j.vetmic.2015.01.016 [doi]
AB  - Infection with equid herpesvirus 1 (EHV-1) may be asymptomatic, or may result in 
      respiratory disease, abortion, neonatal death, or neurological disease. The aim 
      of this study was to estimate the prevalence of EHV-1 infection, including 
      differentiation between genotypes with aspartic acid (D) and asparagine (N) at 
      position 752 of the DNA polymerase sequence, within a selected population of New 
      Zealand horses. The second aim was to determine the predictive value of serology 
      for detection of latently infected horses. Retropharyngeal lymph nodes (RLN) and 
      trigeminal ganglia (TG) were dissected from 52 horses at slaughter and tested for 
      the presence of EHV-1 DNA using magnetic bead, sequence-capture enrichment 
      followed by nested PCR. Sera were tested for EHV-1 antibody using type-specific 
      glycoprotein G ELISA. Overall, 17/52 horses tested positive for EHV-1 DNA. All 
      but one positive PCR results were obtained from RLN samples. Fifteen of the EHV-1 
      positive horses harboured EHV-1 with N752 genotype, one of which was additionally 
      infected with the D752 genotypes of the virus. Our data comprise the first 
      detection of EHV-1 with D752 genotype in New Zealand and suggest that the 
      "neurovirulent" variant of EHV-1 had been present in New Zealand for at least two 
      years before the first reported outbreak of EHM. All sampled horses tested 
      positive for EHV-4 antibody, and 11/52 tested positive for EHV-1 antibody. The 
      strength of agreement between results of EHV-1 PCR and EHV-1 serology was "fair" 
      (Kappa 0.259, 95% CI: -0.022-0.539), which was likely a reflection of low levels 
      of both EHV-1 antibody in sera and EHV-1 DNA in tissues tested.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Dunowska, M
AU  - Dunowska M
AD  - Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 
      Palmerston North, New Zealand. Electronic address: M.Dunowska@massey.ac.nz.
FAU - Gopakumar, G
AU  - Gopakumar G
AD  - Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 
      Palmerston North, New Zealand.
FAU - Perrott, M R
AU  - Perrott MR
AD  - Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 
      Palmerston North, New Zealand.
FAU - Kendall, A T
AU  - Kendall AT
AD  - Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 
      Palmerston North, New Zealand.
FAU - Waropastrakul, S
AU  - Waropastrakul S
AD  - Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 
      Palmerston North, New Zealand.
FAU - Hartley, C A
AU  - Hartley CA
AD  - Centre for Equine Virology, School of Veterinary Science, University of 
      Melbourne, Melbourne, Australia.
FAU - Carslake, H B
AU  - Carslake HB
AD  - Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 
      Palmerston North, New Zealand.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150124
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Disease Outbreaks/*veterinary
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Genotype
MH  - Herpesviridae Infections/*epidemiology/veterinary/virology
MH  - Herpesvirus 1, Equid/genetics/*immunology/isolation & purification
MH  - Herpesvirus 4, Equid/genetics/*immunology/isolation & purification
MH  - Horse Diseases/*epidemiology/virology
MH  - Horses
MH  - New Zealand/epidemiology
MH  - Polymerase Chain Reaction/veterinary
MH  - Pregnancy
MH  - Prevalence
OTO - NOTNLM
OT  - EHV-1
OT  - EHV-1 prevalence
OT  - EHV-1/4 Serology
OT  - Equid herpesvirus 1
OT  - Latency
OT  - PCR
EDAT- 2015/02/11 06:00
MHDA- 2016/05/03 06:00
CRDT- 2015/02/11 06:00
PHST- 2014/10/29 00:00 [received]
PHST- 2015/01/12 00:00 [revised]
PHST- 2015/01/20 00:00 [accepted]
PHST- 2015/02/11 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2016/05/03 06:00 [medline]
AID - S0378-1135(15)00033-4 [pii]
AID - 10.1016/j.vetmic.2015.01.016 [doi]
PST - ppublish
SO  - Vet Microbiol. 2015 Apr 17;176(3-4):219-28. doi: 10.1016/j.vetmic.2015.01.016. 
      Epub 2015 Jan 24.