PMID- 25666563
OWN - NLM
STAT- MEDLINE
DCOM- 20150929
LR  - 20150303
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 87
IP  - 5
DP  - 2015 Mar 3
TI  - Aptamer pseudoknot-functionalized electronic sensor for reagentless and 
      single-step detection of immunoglobulin E in human serum.
PG  - 3094-8
LID - 10.1021/acs.analchem.5b00041 [doi]
AB  - The development of electronic sensors with minimized usage of reagents and 
      washing steps in the sensing protocols will significantly facilitate the 
      detection of biomolecules. In this work, by using a new pseudoknot design of the 
      aptamer probes, the construction of an electronic sensor for reagentless and 
      single-step detection of immunoglobulin E (IgE) in human serum is described. The 
      pseudoknot aptamer probes are self-assembled on the disposable electrode surface. 
      The association of IgE with the aptamer probes leads to conformational changes of 
      the pseudoknot aptamer structures and brings the redox-tags in close proximity to 
      the electrode, resulting in amplified current response for monitoring IgE. The 
      effects of the pseudoknot structure and the immobilization concentration of the 
      aptamer probes on the sensor performance are evaluated. Under optimal conditions, 
      the detection limit for IgE is estimated to be 60 pM. The sensor is also 
      selective and can be employed to detect IgE in human serum samples. The developed 
      sensor can achieve reagentless, washing-free and low-cost (with the disposable 
      electrode) electrochemical detection of proteins, making this device a convenient 
      sensing platform for the monitoring of different biomarkers when coupled with the 
      appropriate aptamer probes.
FAU - Jiang, Bingying
AU  - Jiang B
AD  - School of Chemistry and Chemical Engineering, Chongqing University of Technology 
      , Chongqing 400054, People's Republic of China.
FAU - Li, Fangzhen
AU  - Li F
FAU - Yang, Cuiyun
AU  - Yang C
FAU - Xie, Jiaqing
AU  - Xie J
FAU - Xiang, Yun
AU  - Xiang Y
FAU - Yuan, Ruo
AU  - Yuan R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150219
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques/*methods
MH  - *Electrochemistry
MH  - *Electrodes
MH  - Healthy Volunteers
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Limit of Detection
MH  - Nucleic Acid Conformation
EDAT- 2015/02/11 06:00
MHDA- 2015/09/30 06:00
CRDT- 2015/02/11 06:00
PHST- 2015/02/11 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2015/09/30 06:00 [medline]
AID - 10.1021/acs.analchem.5b00041 [doi]
PST - ppublish
SO  - Anal Chem. 2015 Mar 3;87(5):3094-8. doi: 10.1021/acs.analchem.5b00041. Epub 2015 
      Feb 19.