PMID- 25666734
OWN - NLM
STAT- MEDLINE
DCOM- 20151105
LR  - 20181202
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 115
DP  - 2015 May
TI  - Biochemical responses, morphometric changes, genotoxic effects and CYP1A 
      expression in the armored catfish Pterygoplichthys anisitsi after 15 days of 
      exposure to mineral diesel and biodiesel.
PG  - 26-32
LID - S0147-6513(15)00044-5 [pii]
LID - 10.1016/j.ecoenv.2015.01.034 [doi]
AB  - Despite being considered friendlier to the environment, biodiesel fuel can be 
      harmful to aquatic organisms, especially when combined with petroleum diesel 
      fuel. In this work we evaluated the effects of mineral diesel fuel containing 
      increasing concentrations of biodiesel (5% and 20%, namely B5 and B20) and pure 
      biodiesel (B100), at concentrations of 0.001 and 0.01mLL(-1), after 15 days of 
      exposure, in armored catfish (Pterygoplichtys anisitsi). Toxicity tests were also 
      performed to estimate LC50 values (96h) for each compound. Biotransformation 
      enzymes [ethoxyresorufin-O-deethylase (EROD), and glutathione S-transferase 
      (GST)] as well as oxidative stress markers (superoxide dismutase, SOD, catalase, 
      CAT, glutathione peroxidase, GPx, and the level of lipid peroxidation) were 
      measured in liver and gills after treatment. Genotoxic effects were also accessed 
      in erythrocytes using the comet assay and by evaluating the frequency of 
      micronuclei formation. Further, the mRNA of cytochrome P450 1A (CYP1A) was also 
      measured in liver. Mortality was not observed even exposure to concentrations as 
      high as 6.0mLL(-1). EROD and GST activities were increased after B5 and B20 
      treatments; however, CYP1A mRNA induction was not observed. SOD and CAT 
      activities were decreased, but GPx was significantly higher for all treatments in 
      gills. There were no significant changes in lipid peroxidation, but genotoxicity 
      markers revealed that all treatments increased comet scores. Fuels B5 and B20 
      increased micronuclei frequency. Our results indicate that despite being less 
      toxic, biodiesel may cause sublethal alterations in fish that may alter long term 
      health.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Arantes Felicio, Andreia
AU  - Arantes Felicio A
AD  - Departamento de Quimica e Ciencias Ambientais, IBILCE, UNESP, Sao Jose do Rio 
      Preto, SP, Brasil.
FAU - Martins Parente, Thiago Estevam
AU  - Martins Parente TE
AD  - Laboratorio de Biologia Molecular Ambiental-BioMA, Instituto de Biofisica Carlos 
      Chagas Filho-IBCCF, Universidade Federal do Rio de Janeiro-UFRJ, Brazil.
FAU - Regina Maschio, Lucilene
AU  - Regina Maschio L
AD  - Departamento de Quimica e Ciencias Ambientais, IBILCE, UNESP, Sao Jose do Rio 
      Preto, SP, Brasil.
FAU - Nogueira, Lilian
AU  - Nogueira L
AD  - Departamento de Quimica e Ciencias Ambientais, IBILCE, UNESP, Sao Jose do Rio 
      Preto, SP, Brasil.
FAU - Rodrigues Venancio, Larissa Paola
AU  - Rodrigues Venancio LP
AD  - Centro de Estudo de Quelonios (CEQ) e Laboratorio de Hemoglobinas de Genetica das 
      Doencas Hematologicas (LHGDH), IBILCE, UNESP, Sao Jose do Rio Preto, SP, Brasil.
FAU - de Freitas Rebelo, Mauro
AU  - de Freitas Rebelo M
AD  - Laboratorio de Biologia Molecular Ambiental-BioMA, Instituto de Biofisica Carlos 
      Chagas Filho-IBCCF, Universidade Federal do Rio de Janeiro-UFRJ, Brazil.
FAU - Schlenk, Daniel
AU  - Schlenk D
AD  - Department of Environmental Sciences, University of California, Riverside, 
      3401Watkins Dr, Riverside, CA 92521, USA.
FAU - de Almeida, Eduardo Alves
AU  - de Almeida EA
AD  - Departamento de Quimica e Ciencias Ambientais, IBILCE, UNESP, Sao Jose do Rio 
      Preto, SP, Brasil. Electronic address: ealmeida@ibilce.unesp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150207
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Biofuels)
RN  - 0 (Gasoline)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Biofuels/*toxicity
MH  - Catalase/metabolism
MH  - Catfishes/anatomy & histology/*metabolism
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - Cytochrome P-450 Enzyme System/genetics/*metabolism
MH  - *DNA Damage
MH  - Female
MH  - Gasoline/toxicity
MH  - Gills/drug effects/enzymology
MH  - Glutathione Peroxidase/metabolism
MH  - Glutathione Transferase/metabolism
MH  - Lipid Peroxidation/drug effects
MH  - Liver/drug effects/enzymology
MH  - Male
MH  - Oxidative Stress/drug effects
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - Biodiesel
OT  - Biotransformation enzyme
OT  - CYP1A
OT  - Catfish
OT  - Genotoxicity
OT  - Oxidative stress
EDAT- 2015/02/11 06:00
MHDA- 2015/11/06 06:00
CRDT- 2015/02/11 06:00
PHST- 2014/06/25 00:00 [received]
PHST- 2015/01/26 00:00 [revised]
PHST- 2015/01/31 00:00 [accepted]
PHST- 2015/02/11 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2015/11/06 06:00 [medline]
AID - S0147-6513(15)00044-5 [pii]
AID - 10.1016/j.ecoenv.2015.01.034 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2015 May;115:26-32. doi: 10.1016/j.ecoenv.2015.01.034. 
      Epub 2015 Feb 7.