PMID- 25666955
OWN - NLM
STAT- MEDLINE
DCOM- 20150702
LR  - 20191113
IS  - 0006-9248 (Print)
IS  - 0006-9248 (Linking)
VI  - 116
IP  - 1
DP  - 2015
TI  - Collection of peripheral hematopoietic stem/progenitor cells.
PG  - 9-13
AB  - OBJECTIVES: Several variables possibly affecting collection of peripheral 
      hematopoietic stem/progenitor cells (PBSC) were evaluated: type of apheresis 
      machine (Amicus version 2.5, Baxter vs Cobe Spectra version 7.0, Terumo BCT), 
      venous access (peripheral vein vs central venous catheter, i.g. CVC), and 
      apheresis regimen (standard vs large volume leukapheresis, i.g. SVL vs LVL) with 
      the objective to increase collection efficacy at the site. BACKGROUND: Peripheral 
      blood represents the currently preferred source of hematopoietic stem/progenitor 
      cells (HSCs) for transplantation. METHODS: Data regarding 169 collection 
      procedures performed in healthy donors and patients between January 2008 and 
      December 2011 at the Clinics of Haematology and Transfusiology in St Cyril and 
      Method Hospital in Bratislava (Slovakia) were analysed. RESULTS: With Cobe 
      Spectra apheresis machine it was possible to process larger blood volumes per 
      procedure with higher CD34+ cell collection efficiency (p = 0.0229) and lower RBC 
      contamination of the harvest than with Amicus (p = 0.0116). On the other hand, 
      Amicus helped to limit PLT contamination of the harvest (p < 0.0001), thus 
      minimizing post-procedural decrease in patient s PLT count. The highest detected 
      advantage of CVC usage was higher flow rate of procedure, thus processing larger 
      blood volumes per unit of time. Interesting finding was the tendency to lower 
      harvest PLT contamination (p = 0.054). When LVL was performed, significantly 
      higher HSCs yields were collected, even in "poor mobilizers" when the pre-run 
      parameters were low. CONCLUSION: Management of PBSC collection requires a 
      particular approach in each subject. Institutionally and individually optimized 
      collection may help to improve the transplantation outcome and decrease the 
      financial costs (Tab. 8, Ref. 15).
FAU - Dihenescikova, V Rimajova
AU  - Dihenescikova VR
FAU - Mistrik, M
AU  - Mistrik M
FAU - Martinka, J
AU  - Martinka J
FAU - Zwiewka, M
AU  - Zwiewka M
FAU - Bizikova, I
AU  - Bizikova I
FAU - Batorova, A
AU  - Batorova A
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Bratisl Lek Listy
JT  - Bratislavske lekarske listy
JID - 0065324
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - Hematopoietic Stem Cell Transplantation/*instrumentation/methods
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Leukapheresis/*instrumentation/methods
MH  - Male
MH  - Middle Aged
MH  - Peripheral Blood Stem Cell Transplantation/*instrumentation/methods
MH  - Reference Values
MH  - Slovakia
EDAT- 2015/02/11 06:00
MHDA- 2015/07/03 06:00
CRDT- 2015/02/11 06:00
PHST- 2015/02/11 06:00 [entrez]
PHST- 2015/02/11 06:00 [pubmed]
PHST- 2015/07/03 06:00 [medline]
AID - 10.4149/bll_2015_002 [doi]
PST - ppublish
SO  - Bratisl Lek Listy. 2015;116(1):9-13. doi: 10.4149/bll_2015_002.