PMID- 25669596
OWN - NLM
STAT- MEDLINE
DCOM- 20151214
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 176
IP  - 3-4
DP  - 2015 Apr 17
TI  - Characterization of two novel porcine reproductive and respiratory syndrome virus 
      isolates with deletions in the GP2 gene.
PG  - 344-51
LID - S0378-1135(15)00035-8 [pii]
LID - 10.1016/j.vetmic.2015.01.018 [doi]
AB  - Two newly emerged, porcine reproductive and respiratory syndrome virus (PRRSV) 
      strains (Henan-A10 and A11) were isolated from the sera of aborting sows. 
      Interestingly, both of the isolates could replicate in primary porcine alveolar 
      macrophage (PAM) cells but not in MARC-145 cells. A phylogenetic tree based on 
      the complete genome was constructed and the results showed that Henan-A10 and A11 
      were most closely related to other highly pathogenic PRRSV (HP-PRRSV) strains. 
      However, genomic sequence analysis showed that Henan-A10 and A11 shared only 
      96.8-97.8% nucleotide identity with the representative HP-PRRSV strain JXA1. 
      Notably, a 10 amino acids deletion in the GP2 endodomain was identified for the 
      first time. A full-length, infectious cDNA clone of HuN4-F112 (attenuated strain 
      from a HP-PRRSV) was used to construct a chimeric clone with the corresponding 
      deletion in GP2. We found that the deletion did not affect viral growth in 
      MARC-145 cells, indicating that the endodomain of PRRSV GP2 may be variable.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Chen, Jia-zeng
AU  - Chen JZ
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Peng, Jin-mei
AU  - Peng JM
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Bai, Yun
AU  - Bai Y
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Wang, Qian
AU  - Wang Q
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Liu, Yi-min
AU  - Liu YM
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Zhang, Qiu-yue
AU  - Zhang QY
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Chang, Dan
AU  - Chang D
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Zhang, Wu-chao
AU  - Zhang WC
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Zhao, Hong-yuan
AU  - Zhao HY
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Ye, Chao
AU  - Ye C
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - An, Tong-qing
AU  - An TQ
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Cai, Xue-hui
AU  - Cai XH
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Tian, Zhi-jun
AU  - Tian ZJ
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. 
      Electronic address: tzj@hvri.ac.cn.
FAU - Tong, Guang-zhi
AU  - Tong GZ
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China. Electronic address: gztong@shvri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150128
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Genome, Viral/*genetics
MH  - Genomics
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Sequence Analysis
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
MH  - Swine
OTO - NOTNLM
OT  - GP2
OT  - Genetic diversity
OT  - PRRSV
EDAT- 2015/02/12 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/02/12 06:00
PHST- 2014/05/22 00:00 [received]
PHST- 2014/12/15 00:00 [revised]
PHST- 2015/01/20 00:00 [accepted]
PHST- 2015/02/12 06:00 [entrez]
PHST- 2015/02/12 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0378-1135(15)00035-8 [pii]
AID - 10.1016/j.vetmic.2015.01.018 [doi]
PST - ppublish
SO  - Vet Microbiol. 2015 Apr 17;176(3-4):344-51. doi: 10.1016/j.vetmic.2015.01.018. 
      Epub 2015 Jan 28.