PMID- 25670712
OWN - NLM
STAT- MEDLINE
DCOM- 20160209
LR  - 20220318
IS  - 1471-9053 (Electronic)
IS  - 0032-0781 (Linking)
VI  - 56
IP  - 5
DP  - 2015 May
TI  - Epigenetic Changes are Associated with Programmed Cell Death Induced by Heat 
      Stress in Seedling Leaves of Zea mays.
PG  - 965-76
LID - 10.1093/pcp/pcv023 [doi]
AB  - Histone modification plays a crucial role in regulation of chromatin architecture 
      and function, responding to adverse external stimuli. However, little is known 
      about a possible relationship between epigenetic modification and programmed cell 
      death (PCD) in response to environmental stress. Here, we found that heat stress 
      induced PCD in maize seedling leaves which was characterized by chromatin DNA 
      laddering and DNA strand breaks detected by a terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) test. The activities of the 
      reactive oxygen species (ROS)-related enzymes superoxide dismutase (SOD), 
      peroxidase (POD) and catalase (CAT) were progressively increased over time in the 
      heat-treated seedlings. However, the concentration of H2O2 remained at relatively 
      lower levels, while the concentration of superoxide anion ([Formula: see text]) 
      was increased, accompanied by the occurrence of higher ion leakage rates after 
      heat treatment. The total acetylation levels of histones H3K9, H4K5 and H3 were 
      significantly increased, whereas the di-methylation level of histone H3K4 was 
      unchanged and the di-methylation level of histone H3K9 was decreased in the 
      seedling leaves exposed to heat stress compared with the control seedlings, 
      accompanied by increased nucleolus size indicative of chromatin decondensation. 
      Furthermore, treatment of seedlings with trichostatin A (TSA), which always 
      results in genomic histone hyperacetylation, caused an increase in the [Formula: 
      see text] level within the cells. The results suggested that heat stress 
      persistently induced [Formula: see text], leading to PCD in association with 
      histone modification changes in the maize leaves.
CI  - (c) The Author 2015. Published by Oxford University Press on behalf of Japanese 
      Society of Plant Physiologists. All rights reserved. For permissions, please 
      email: journals.permissions@oup.com.
FAU - Wang, Pu
AU  - Wang P
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Zhao, Lin
AU  - Zhao L
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Hou, Haoli
AU  - Hou H
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Huang, Yan
AU  - Huang Y
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Wang, Yapei
AU  - Wang Y
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Li, Hui
AU  - Li H
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Gao, Fei
AU  - Gao F
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Yan, Shihan
AU  - Yan S
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China.
FAU - Li, Lijia
AU  - Li L
AD  - State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 
      Wuhan 430072, China ljli@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150209
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 0 (Chromatin)
RN  - 0 (Histones)
RN  - 0 (Hydroxamic Acids)
RN  - 0 (Ions)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 3X2S926L3Z (trichostatin A)
SB  - IM
MH  - Acetylation/drug effects
MH  - Apoptosis/drug effects/*genetics
MH  - Blotting, Western
MH  - Chromatin/metabolism
MH  - DNA Damage
MH  - *Epigenesis, Genetic/drug effects
MH  - Exons/genetics
MH  - Gene Expression Regulation, Plant/drug effects
MH  - Genes, Plant
MH  - Heat-Shock Response/*genetics
MH  - Histones/metabolism
MH  - Hydroxamic Acids/pharmacology
MH  - In Situ Nick-End Labeling
MH  - Ions
MH  - Methylation/drug effects
MH  - Models, Biological
MH  - Plant Leaves/*cytology/drug effects/genetics
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Seedlings/cytology/drug effects/*genetics
MH  - Stress, Physiological/drug effects/*genetics
MH  - Zea mays/drug effects/enzymology/*genetics/physiology
OTO - NOTNLM
OT  - Heat stress
OT  - Histone modification
OT  - Maize seedlings
OT  - PCD
OT  - Superoxide anion
EDAT- 2015/02/12 06:00
MHDA- 2016/02/10 06:00
CRDT- 2015/02/12 06:00
PHST- 2014/09/30 00:00 [received]
PHST- 2015/02/04 00:00 [accepted]
PHST- 2015/02/12 06:00 [entrez]
PHST- 2015/02/12 06:00 [pubmed]
PHST- 2016/02/10 06:00 [medline]
AID - pcv023 [pii]
AID - 10.1093/pcp/pcv023 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2015 May;56(5):965-76. doi: 10.1093/pcp/pcv023. Epub 2015 Feb 
      9.