PMID- 25671
OWN - NLM
STAT- MEDLINE
DCOM- 19780724
LR  - 20081121
IS  - 0006-3029 (Print)
IS  - 0006-3029 (Linking)
VI  - 23
IP  - 2
DP  - 1978 Mar-Apr
TI  - [Reaction between polycytidylic acid and guanosine-5'-triphosphate at neural pH].
PG  - 208-12
AB  - The effect of changing the solution acidity in the interval of pH 7--8 on complex 
      formation between polycytidilyc acid (polyC) and guanosine-5'-triphosphate (GTP) 
      has been studied by the equilibrium dialysis and hydrogen ion titration methods. 
      It is shown that in 1 M NaCl at 3 degrees C the complex stoichiometry undergoes 
      changes in a narrow pH interval being equal to 2 poly C: 1 GTP at pH 7,0--7,3 and 
      to 1 poly C:1 GTP at pH 7,6--8,0. In the presence of GTP the apparent pK of poly 
      C protonation increases by 1,4 PK units and becomes equal to 7,1 in 1 M NaCl at 3 
      degrees C. Thus, the protonated poly-C takes part in the three-stranded 
      poly-C--GTP complex formation. According to Hill's model of cooperative linear 
      adsorption it is shown that the cooperativity of the three-stranded complex 
      formation is greater than of the two-stranded one. The possibility of drastic 
      changes in the structure of biopolymers with small pH changes in neutral region 
      we have found for the poly-C--GTP system as an example may play an essential role 
      in matrix synthesis and other intracellular processes.
FAU - Gukovskaia, A S
AU  - Gukovskaia AS
FAU - Lanin, V N
AU  - Lanin VN
FAU - Sukhorukov, B I
AU  - Sukhorukov BI
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Issledovanie vzaimodeistviia politsitilovoi kisloty s guanozin-5'-trifosfatom pri 
      neitral'nykh pH.
PL  - Russia (Federation)
TA  - Biofizika
JT  - Biofizika
JID - 0372666
RN  - 0 (Polyribonucleotides)
RN  - 30811-80-4 (Poly C)
RN  - 86-01-1 (Guanosine Triphosphate)
SB  - IM
MH  - Chemical Phenomena
MH  - Chemistry
MH  - *Guanosine Triphosphate
MH  - Hydrogen-Ion Concentration
MH  - *Poly C
MH  - *Polyribonucleotides
EDAT- 1978/03/01 00:00
MHDA- 1978/03/01 00:01
CRDT- 1978/03/01 00:00
PHST- 1978/03/01 00:00 [pubmed]
PHST- 1978/03/01 00:01 [medline]
PHST- 1978/03/01 00:00 [entrez]
PST - ppublish
SO  - Biofizika. 1978 Mar-Apr;23(2):208-12.