PMID- 25672412
OWN - NLM
STAT- MEDLINE
DCOM- 20160204
LR  - 20220409
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 33
IP  - 4
DP  - 2015 Apr
TI  - Overexpression of Tiam1 promotes the progression of laryngeal squamous cell 
      carcinoma.
PG  - 1807-14
LID - 10.3892/or.2015.3785 [doi]
AB  - T-lymphoma invasion and metastasis‑inducing factor 1 (Tiam1) has been reported in 
      various types of human cancer, which play important roles in facilitating the 
      meta-stasis of malignant tumor. However, the investigation of Tiam1 in laryngeal 
      squamous-cell carcinoma is extremely rare. The aim of the present study was to 
      assess Tiam1 expression and examine its function in tumorigenesis and the 
      metastasis of laryngeal squamous cell carcinoma (LSCC) in vitro. Tiam1 expression 
      in 98 primary LSCC tissue specimens was analyzed by immunohistochemistry and 
      correlated with clinicopathological parameters and patients' survival. To 
      investigate the effects of Tiam1 on the progression of LSCC, Tiam1/C1199 plasmid 
      was transfected into LSCC, and proliferation, apoptosis, migration and invasion 
      of transfected cells were examined using MTT, flow cytometry, wound-healing and 
      Transwell assay, respectively. The results showed that, Tiam1 was detected in all 
      primary LSCC samples. Additionally, Tiam1 overexpression was closely correlated 
      with tumor progression and patient survival. Tiam1 overexpression was 
      statistically significant, and served as an independent predictor of prognosis 
      for patients with LSCC. The upregulation of Tiam1 by Tiam1/C1199 plasmid had no 
      effect on the prolife-ration of transfected cells, but decreased the apoptotic 
      rate of transfected cells, while the ability of migration and invasion was 
      increased. These results suggested that Tiam1 overexpression in LSCC is possibly 
      involved in the promotion of migration and invasion, and is a promising 
      therapeutic target in the prevention of the progression of LSCC.
FAU - Wang, Shuang
AU  - Wang S
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University, Changsha, Hunan 410011, P.R. China.
FAU - Li, Shisheng
AU  - Li S
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University, Changsha, Hunan 410011, P.R. China.
FAU - Tang, Qinglai
AU  - Tang Q
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University, Changsha, Hunan 410011, P.R. China.
FAU - Yang, Shu
AU  - Yang S
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University, Changsha, Hunan 410011, P.R. China.
FAU - Wang, Shuhui
AU  - Wang S
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University, Changsha, Hunan 410011, P.R. China.
FAU - Liu, Jiajia
AU  - Liu J
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University, Changsha, Hunan 410011, P.R. China.
FAU - Yang, Mi
AU  - Yang M
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University, Changsha, Hunan 410011, P.R. China.
FAU - Yang, Xinming
AU  - Yang X
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University, Changsha, Hunan 410011, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20150205
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (T-Lymphoma Invasion and Metastasis-inducing Protein 1)
RN  - 0 (TIAM1 protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*diagnosis/*genetics
MH  - Cell Movement
MH  - Disease Progression
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Neoplastic/*physiology
MH  - Guanine Nucleotide Exchange Factors/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*diagnosis/*genetics
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Plasmids
MH  - Prognosis
MH  - Retrospective Studies
MH  - T-Lymphoma Invasion and Metastasis-inducing Protein 1
MH  - Transfection/methods
MH  - Young Adult
EDAT- 2015/02/13 06:00
MHDA- 2016/02/05 06:00
CRDT- 2015/02/13 06:00
PHST- 2014/12/03 00:00 [received]
PHST- 2015/01/21 00:00 [accepted]
PHST- 2015/02/13 06:00 [entrez]
PHST- 2015/02/13 06:00 [pubmed]
PHST- 2016/02/05 06:00 [medline]
AID - 10.3892/or.2015.3785 [doi]
PST - ppublish
SO  - Oncol Rep. 2015 Apr;33(4):1807-14. doi: 10.3892/or.2015.3785. Epub 2015 Feb 5.