PMID- 25672467
OWN - NLM
STAT- MEDLINE
DCOM- 20150324
LR  - 20181113
IS  - 1330-0962 (Print)
IS  - 1846-7482 (Electronic)
IS  - 1330-0962 (Linking)
VI  - 25
IP  - 1
DP  - 2015
TI  - Comparison of blood ethanol stabilities in different storage periods.
PG  - 57-63
LID - 10.11613/BM.2015.006 [doi]
AB  - INTRODUCTION: Measurements of blood ethanol concentrations must be accurate and 
      reliable. The most important factors affecting blood ethanol stability are 
      temperature and storage time. In this study, we aimed to compare ethanol 
      stability in plasma samples at -20  degrees C for the different storage periods. 
      MATERIALS AND METHODS: Blood samples were collected from intoxicated drivers 
      (N=80) and initial plasma ethanol concentrations were measured immediately. 
      Plasma samples were then stored at -20  degrees C and re-assessed after 2, 3, 4, or 5 
      months of storage. Differences between the initial and stored ethanol 
      concentrations in each group (N=20) were analyzed using Wilcoxon matched-pairs 
      test. The deviation from the initial concentration was calculated and compared 
      with Clinical Laboratory Improvement Amendments (CLIA'88) Proficiency Testing 
      Limits. Relationships between the initial concentrations and deviations from 
      initial concentrations were analyzed by Spearman's correlation analysis. For all 
      statistical tests, differences with P values of less than 0.05 were considered 
      statistically significant. RESULTS: Statistically significant differences were 
      observed between the initial and poststorage ethanol concentrations in the 
      overall sample group (P<0.001). However, for the individual storage duration 
      groups, analytically significant decreases were observed only for samples stored 
      for 5 months, deviations from the initial concentrations exceeded the allowable 
      total error (TEa). Ethanol decreases in the other groups did not exceed the TEa. 
      CONCLUSION: According to our results, plasma ethanol samples can be kept at -20 
       degrees C for up to 3-4 months until re-analysis. However, each laboratory should also 
      establish its own work-flow rules and criterion for reliable ethanol measurement 
      in forensic cases.
FAU - Kocak, Fatma Emel
AU  - Kocak FE
AD  - Dumlupinar University, Faculty of Medicine, Department of Medical Biochemistry, 
      Kutahya, Turkey.
FAU - Isiklar, Ozben Ozden
AU  - Isiklar OO
AD  - Dumlupinar University, Evliya Celebi Education, and Research Hospital, Department 
      of Medical Biochemistry, Kutahya, Turkey.
FAU - Kocak, Havva
AU  - Kocak H
AD  - Dumlupinar University, Faculty of Medicine, Department of Medical Biochemistry, 
      Kutahya, Turkey.
FAU - Meral, Ayfer
AU  - Meral A
AD  - Dumlupinar University, Evliya Celebi Education, and Research Hospital, Department 
      of Medical Biochemistry, Kutahya, Turkey.
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - Croatia
TA  - Biochem Med (Zagreb)
JT  - Biochemia medica
JID - 9610305
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Alcoholic Intoxication/*blood
MH  - *Automobile Driving
MH  - Cryopreservation/*methods
MH  - Ethanol/*blood
MH  - Female
MH  - Humans
MH  - Male
MH  - Reproducibility of Results
MH  - Time Factors
PMC - PMC4401314
OTO - NOTNLM
OT  - ethanol
OT  - preanalytical phase
OT  - stability
OT  - storage temperature
COIS- None declared.
EDAT- 2015/02/13 06:00
MHDA- 2015/03/25 06:00
PMCR- 2015/02/15
CRDT- 2015/02/13 06:00
PHST- 2014/06/29 00:00 [received]
PHST- 2014/11/29 00:00 [accepted]
PHST- 2015/02/13 06:00 [entrez]
PHST- 2015/02/13 06:00 [pubmed]
PHST- 2015/03/25 06:00 [medline]
PHST- 2015/02/15 00:00 [pmc-release]
AID - biomd-25-1-57 [pii]
AID - BM_25(1)_57-63 [pii]
AID - 10.11613/BM.2015.006 [doi]
PST - ppublish
SO  - Biochem Med (Zagreb). 2015;25(1):57-63. doi: 10.11613/BM.2015.006.