PMID- 25675573
OWN - NLM
STAT- MEDLINE
DCOM- 20150324
LR  - 20150213
IS  - 0255-2930 (Print)
IS  - 0255-2930 (Linking)
VI  - 34
IP  - 11
DP  - 2014 Nov
TI  - [Effects of electroacupuncture at "Yishu" (EX-B 3) on the relative hormones of 
      HPA axis in rats with type-2 diabetes mellitus].
PG  - 1099-105
AB  - OBJECTIVE: To observe the intervention effects of electroacupuncture at "Yishu" 
      (EX-B 3) on rats with type-2 diabetes mellitus (T2DM), so as to provide 
      experiment references for acupuncture to treat T2DM. METHODS: Among seventy male 
      Wistar clean-grade rats, 8 rats were randomly selected into a control group; the 
      rest rats were made T2DM model. Fifty-two rats which were successfully made T2DM 
      model, according to randomized block method, were divided into a model group (10 
      rats), a medication group (10 rats), an electroacupuncture at "Shenshu" (BL 23) 
      group (11 rats), an electroacupuncture at "Pishu" (BL 20) group (10 rats) and an 
      electroacupuncture at "Yishu" (EX-B 3) group (11 rats). Seven days after 
      successful establishment of model, the rats in the model group were fixed in the 
      self-made rat bag without receiving any treatment; the rats in the medication 
      group, according to body mass (10 mL/kg), were treated with intragastric 
      administration of glimepiride; the rats in all the electroacupuncture groups were 
      treated with electroacupuncture at "Shenshu" (BL 23), "Pishu" (BL 20) and "Yishu" 
      (EX-B 3), respectively. The continuous wave was selected with a frequency of 15 
      Hz and a current intensity of 4 to 6 mA. The treatment was given 20 min per 
      treatment, once a day, 5 treatments per week for continuous 4 weeks. Before the 
      establishment of model and continuous 4 weeks after the intervention, blood 
      samples were collected from rats' caudal vein, and fasting blood glucose (FBG) 
      was measured with FBG device each week. After the last intervention, the rats 
      were killed and hypothalamus, pituitary and adrenal gland were collected. The 
      colorimetric method was applied to measure the contents of triglyceride (TG), 
      cholesterol (TC), high-density lipoprotein (HDL-C) and low-density lipoprotein 
      (LDL-C); radioimmunoassay was used to test the contents of glycated serum protein 
      (GSP), fasting insulin (FINS), corticotropin-releasing hormone (CRH), 
      adrenocorticotropic hormone (ACTH) and cortin (CORT). RESULTS: Four weeks after 
      the intervention, except that the rat's body mass in the normal group continued 
      to increase, body mass in the model group, medication group and each 
      electroacupuncture group were significantly reduced (P<0.01, P<0.05). Compared 
      with the model group, the FBG in the electroacupuncture at "Pishu" (BL 20) group 
      and electroacupuncture at "Yishu" (EX-B 3) group were obviously reduced (P<0.05, 
      P<0.01); FBG in the electroacupuncture at "Yishu" (EX-B 3) group was lower than 
      that in the medication group and electroacupuncture at "Shenshu" (BL 23) group 
      (both P<0.05). The contents of TG, HDL-C and LDL-C in the electroacupuncture at 
      "Yishu" (EX-B 3) group were reduced (P<0.05, P<0.01), the content of TG was 
      significantly lower than that in the medication group and electroacupuncture at 
      "Shenshu" (BL 23) group (both P<0.05), the content of LDL-C was significantly 
      lower than that in electroacupuncture at "Shenshu" (BL 23) group (P<0.05). 
      Insulin sensitivity index (ISI) in the medication group, electroacupuncture at 
      "Pishu" (BL 20) group and electroacupuncture at "Yishu (EX-B 3)" group were 
      evidently increased (P<0.05, P<0.01); ISI in the medication group was lower than 
      that in the electroacupuncture at "Yishu" (EX-B 3) group (P<0.05). The content of 
      CRH in the electroacupuncture at "Yishu" (EX-B 3) group was lower than that in 
      the medication group and electroacupuncture at "Shenshu" (BL 23) group (P<0.05, 
      P<0.01); the content of CORT in the electroacupuncture at "Yishu" (EX-B 3) group 
      was lower than that in the medication group and electroacupuncture at "Pishu" (BL 
      20) group (both P<0.05). CONCLUSION: Electroacupuncture at "Yishu" (EX-B 3) could 
      reduce the level of CORT to improve the insulin resistance in rats with T2DM, 
      improve insulin sensitivity index, regulate blood lipid metabolism and relieve 
      the hyperactivity of the HPA axis.
FAU - Gao, Shana
AU  - Gao S
FAU - Li, Rui
AU  - Li R
FAU - Tian, Huan-huan
AU  - Tian HH
FAU - Pei, En-shi
AU  - Pei ES
FAU - Cao, Bing-yan
AU  - Cao BY
FAU - Wu, Yan
AU  - Wu Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhen Jiu
JT  - Zhongguo zhen jiu = Chinese acupuncture & moxibustion
JID - 8600658
RN  - 0 (Blood Glucose)
RN  - 0 (Hormones)
SB  - IM
MH  - *Acupuncture Points
MH  - *Acupuncture Therapy
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Diabetes Mellitus, Type 2/metabolism/*therapy
MH  - *Electroacupuncture
MH  - Hormones/*metabolism
MH  - Humans
MH  - Hypothalamus/*metabolism
MH  - Male
MH  - Pituitary-Adrenal System/*metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2015/02/14 06:00
MHDA- 2015/03/25 06:00
CRDT- 2015/02/14 06:00
PHST- 2015/02/14 06:00 [entrez]
PHST- 2015/02/14 06:00 [pubmed]
PHST- 2015/03/25 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhen Jiu. 2014 Nov;34(11):1099-105.