PMID- 25675919
OWN - NLM
STAT- MEDLINE
DCOM- 20151117
LR  - 20150214
IS  - 1875-6263 (Electronic)
IS  - 1028-4559 (Linking)
VI  - 54
IP  - 1
DP  - 2015 Feb
TI  - The efficiency of conventional microscopic selection is comparable to the 
      hyaluronic acid binding method in selecting spermatozoa for male infertility 
      patients.
PG  - 48-53
LID - S1028-4559(14)00206-X [pii]
LID - 10.1016/j.tjog.2014.11.006 [doi]
AB  - OBJECTIVE: To evaluate if hyaluronic acid (HA)-bound spermatozoa surpassed 
      conventional microscopy-selected spermatozoa in the status of sperm DNA integrity 
      by acridine orange (AO) fluorescence staining. MATERIALS AND METHODS: Spermatozoa 
      obtained from couples with indication for the intracytoplasmic sperm injection 
      (ICSI) procedure due to male infertility (n = 34) and control males with normal 
      sperm parameters (n = 12) were analyzed using AO fluorescence staining after 
      density-gradient centrifugation (DGC), polyvinylpyrrolidone (PVP)-microscopic 
      selection, and HA-binding selection to determine sperm DNA integrity. RESULTS: 
      Percentages of DNA intact spermatozoa with green fluorescence were significantly 
      higher in both PVP-microscopic selected spermatozoa (82.1 +/- 24.0%) and HA-bound 
      spermatozoa (83.9 +/- 21.1%) than in spermatozoa prepared by DGC (66.8 +/- 24.0%). 
      However, there was no significant difference between the PVP-sperm and HA-sperm 
      groups. When the percentage of green fluorescent spermatozoa prepared by DGC fell 
      initially below 68%, both PVP-microscopic and HA-binding selection failed to 
      select over 90% spermatozoa with intact DNA for ICSI in the male infertility 
      group. Compared to control males with normal sperm parameters (99.3 +/- 1.8%), the 
      proportion of green fluorescence sperm after HA-binding selection from couples 
      with male infertility (83.9 +/- 21.1%) did not reach the range of > 99% reported by 
      Yagci et al. CONCLUSION: The percentages of DNA intact spermatozoa between the 
      PVP-sperm and HA-sperm groups were not significantly different. In an ICSI 
      procedure, a well-trained embryologist will have the same ability to choose sperm 
      with intact DNA by conventional microscopic selection as with HA-bound 
      spermatozoa selection.
CI  - Copyright (c) 2014. Published by Elsevier B.V.
FAU - Huang, Meng-Ting
AU  - Huang MT
AD  - Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, 
      Taiwan.
FAU - Kuo-Kuang Lee, Robert
AU  - Kuo-Kuang Lee R
AD  - Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, 
      Taiwan; Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan; 
      Department of Obstetrics and Gynecology, Taipei Medical University, Taipei, 
      Taiwan.
FAU - Lu, Chung-Hao
AU  - Lu CH
AD  - Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan.
FAU - Chen, Ying-Jie
AU  - Chen YJ
AD  - Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, 
      Taiwan.
FAU - Li, Sheng-Hsiang
AU  - Li SH
AD  - Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan; Mackay 
      Medicine, Nursing and Management College, Taipei, Taiwan.
FAU - Hwu, Yuh-Ming
AU  - Hwu YM
AD  - Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, 
      Taiwan; Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan; 
      Mackay Medicine, Nursing and Management College, Taipei, Taiwan; Mackay Medical 
      College, Taipei, Taiwan. Electronic address: hwu4416@yahoo.com.tw.
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Taiwan J Obstet Gynecol
JT  - Taiwanese journal of obstetrics & gynecology
JID - 101213819
RN  - 0 (Viscosupplements)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Adult
MH  - DNA Fragmentation/drug effects
MH  - Embryo Implantation
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Hyaluronic Acid/*administration & dosage
MH  - Infertility, Male/diagnosis/*drug therapy/genetics
MH  - Male
MH  - Retrospective Studies
MH  - Sperm Injections, Intracytoplasmic/*methods
MH  - Sperm Retrieval
MH  - Spermatozoa/cytology/*drug effects/physiology
MH  - Treatment Outcome
MH  - Viscosupplements/administration & dosage
OTO - NOTNLM
OT  - PVP-ICSI
OT  - acridine orange fluorescence test
OT  - hyaluronic acid selection
OT  - sperm DNA integrity
OT  - sperm selection
EDAT- 2015/02/14 06:00
MHDA- 2015/11/18 06:00
CRDT- 2015/02/14 06:00
PHST- 2014/03/07 00:00 [accepted]
PHST- 2015/02/14 06:00 [entrez]
PHST- 2015/02/14 06:00 [pubmed]
PHST- 2015/11/18 06:00 [medline]
AID - S1028-4559(14)00206-X [pii]
AID - 10.1016/j.tjog.2014.11.006 [doi]
PST - ppublish
SO  - Taiwan J Obstet Gynecol. 2015 Feb;54(1):48-53. doi: 10.1016/j.tjog.2014.11.006.