PMID- 25677371
OWN - NLM
STAT- MEDLINE
DCOM- 20151202
LR  - 20171116
IS  - 1521-3765 (Electronic)
IS  - 0947-6539 (Linking)
VI  - 21
IP  - 12
DP  - 2015 Mar 16
TI  - Tris-functionalized hybrid Anderson polyoxometalates: synthesis, 
      characterization, hydrolytic stability and inversion of protein surface charge.
PG  - 4762-71
LID - 10.1002/chem.201405644 [doi]
AB  - Single- and double-sided functionalized hybrid organic-inorganic Anderson 
      polyoxomolybdates with Ga(III) and Fe(III) positioned as central heteroatoms have 
      been synthesized in a mild, two-step synthesis in an aqueous medium. Compounds 
      1-4 were isolated as hydrated salts, [TBA]3[GaMo6O18(OH)3(OCH2)3CCH2OH]x12 H2O 
      (1) (TBA = tetrabutylammonium), Na3[FeMo6O18(OCH2)3CCH2OH2]x11 H2O (2), 
      [TMA]2[GaMo6O18(OH)3(OCH2)3CNH3]x7 H2O (3) (TMA = tetramethylammonium), and 
      Na[TMA]2[FeMo6O18(OH)3(OCH2)3CNH3](OH)x6 H2O (4). All the compounds were 
      characterized based on single-crystal X-ray diffraction (SXRD), FTIR, UV/Vis, 
      thermogravimetric, ESI-MS, NMR, and elemental analyses. Compound 1 was also 
      crystallized with two smaller organic cations, giving 
      [TMA]3[GaMo6O18(OH)3(OCH2)3CCH2OH]xn H2O (5) and 
      [GDM]3[GaMo6O18(OH)3(OCH2)3CCH2OH]xn H2O (6) (GDM = guanidinium) and were 
      characterized based on UV/Vis, NMR, FTIR, and elemental analyses. The use of 
      these compounds as additives in macromolecular crystallography was investigated 
      by examining their hydrolytic stability by using ESI-MS in a pH range of 4 to 9. 
      Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis 
      showed that BSA remains intact in a solution containing up to 100 equivalents of 
      1 or 4 over more than four days at 20  degrees C. Zeta potential measurements demonstrate 
      that 1-4 induce charge inversions on the positively charged surface of BSA 
      (1 mg mL(-1)) with concentrations starting as low as 1.29 mM for compounds 1 and 
      2, which have the highest negative surface charge.
CI  - (c) 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Blazevic, Amir
AU  - Blazevic A
AD  - Institut fur Biophysikalische Chemie, Fakultat fur Chemie, Universitat Wien, 
      Althanstrasse 14, 1090 Wien (Austria).
FAU - Al-Sayed, Emir
AU  - Al-Sayed E
FAU - Roller, Alexander
AU  - Roller A
FAU - Giester, Gerald
AU  - Giester G
FAU - Rompel, Annette
AU  - Rompel A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150210
PL  - Germany
TA  - Chemistry
JT  - Chemistry (Weinheim an der Bergstrasse, Germany)
JID - 9513783
RN  - 0 (Tungsten Compounds)
RN  - 0 (polyoxometalate I)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Crystallography, X-Ray
MH  - Hydrolysis
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Conformation
MH  - Serum Albumin, Bovine/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Thermogravimetry
MH  - Tungsten Compounds/chemical synthesis/*chemistry
MH  - X-Ray Absorption Spectroscopy
OTO - NOTNLM
OT  - POM-protein interactions
OT  - crystal growth
OT  - organic-inorganic hybrid composites
OT  - polyoxometalates
EDAT- 2015/02/14 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/02/14 06:00
PHST- 2014/10/14 00:00 [received]
PHST- 2015/02/14 06:00 [entrez]
PHST- 2015/02/14 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1002/chem.201405644 [doi]
PST - ppublish
SO  - Chemistry. 2015 Mar 16;21(12):4762-71. doi: 10.1002/chem.201405644. Epub 2015 Feb 
      10.