PMID- 25678460
OWN - NLM
STAT- MEDLINE
DCOM- 20160307
LR  - 20181113
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 51
IP  - 6
DP  - 2015 Jun
TI  - Caloric restriction mimetic 2-deoxyglucose maintains cytoarchitecture and reduces 
      tau phosphorylation in primary culture of mouse hippocampal pyramidal neurons.
PG  - 546-55
LID - 10.1007/s11626-015-9867-1 [doi]
AB  - Typical form of neurons is crucially important for their functions. This is 
      maintained by microtubules and associated proteins like tau. Hyperphosphorylation 
      of tau is a major concern in neurodegenerative diseases. Glycogen synthase 
      kinase3beta (GSK3beta) and cyclin-dependent protein kinase 5 (Cdk5) are the enzymes 
      that govern tau phosphorylation. Currently, efforts are being made to target 
      GSK3beta and Cdk5 as possible therapeutic avenues to control tau phosphorylation and 
      treat neurodegenerative diseases related to taupathies. In a number of studies, 
      caloric restriction mimetic 2-deoxyglucose (C6H12O5) was found to be beneficial 
      in improving the brain functions. However, no reports are available on the effect 
      of 2-deoxyglucose 2-DG on tau phosphorylation. In the present study, hippocampal 
      pyramidal neurons from E17 mouse embryos were isolated and cultured on 
      poly-L-lysine-coated coverslips. Neurons from the experimental group were treated 
      with 10 mM 2-deoxyglucose. The treatment of 2-DG resulted in healthier neuronal 
      morphology in terms of significantly lower number of cytoplasmic vacuoles, little 
      or no membrane blebbings, maintained axon hillock and intact neurites. There were 
      decreased immunofluorescence signals for GSK3beta, pTau at Ser262, Cdk5 and pTau at 
      Ser235 suggesting decreased tau phosphorylation, which was further confirmed by 
      Western blotting. The results indicate the beneficial effects of 2-DG in 
      controlling the tau phosphorylation and maintaining the healthy neuronal 
      cytoarchitecture.
FAU - Bele, M S
AU  - Bele MS
AD  - Cellular stress response laboratory, Cell Biology Division, Department of 
      Zoology, Shivaji University, Kolhapur, India.
FAU - Gajare, K A
AU  - Gajare KA
FAU - Deshmukh, A A
AU  - Deshmukh AA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150213
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Mtap2 protein, mouse)
RN  - 0 (tau Proteins)
RN  - 9G2MP84A8W (Deoxyglucose)
RN  - EC 2.7.11.1 (Cyclin-Dependent Kinase 5)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.11.1 (Gsk3b protein, mouse)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - *Caloric Restriction
MH  - Cells, Cultured
MH  - Cyclin-Dependent Kinase 5/metabolism
MH  - Deoxyglucose/*pharmacology
MH  - Fluorescent Antibody Technique
MH  - Glycogen Synthase Kinase 3/metabolism
MH  - Glycogen Synthase Kinase 3 beta
MH  - Hippocampus/*cytology
MH  - Mice
MH  - Microtubule-Associated Proteins/metabolism
MH  - Phosphorylation/drug effects
MH  - Pyramidal Cells/*cytology/drug effects/*metabolism
MH  - tau Proteins/*metabolism
EDAT- 2015/02/14 06:00
MHDA- 2016/03/08 06:00
CRDT- 2015/02/14 06:00
PHST- 2014/08/25 00:00 [received]
PHST- 2015/01/01 00:00 [accepted]
PHST- 2015/02/14 06:00 [entrez]
PHST- 2015/02/14 06:00 [pubmed]
PHST- 2016/03/08 06:00 [medline]
AID - 10.1007/s11626-015-9867-1 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2015 Jun;51(6):546-55. doi: 
      10.1007/s11626-015-9867-1. Epub 2015 Feb 13.