PMID- 25678530
OWN - NLM
STAT- MEDLINE
DCOM- 20150821
LR  - 20221207
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 96
IP  - Pt 6
DP  - 2015 Jun
TI  - Hantavirus-induced pathogenesis in mice with a humanized immune system.
PG  - 1258-1263
LID - 10.1099/vir.0.000087 [doi]
AB  - Hantaviruses are emerging zoonotic pathogens that can cause severe disease in 
      humans. Clinical observations suggest that human immune components contribute to 
      hantavirus-induced pathology. To address this issue we generated mice with a 
      humanized immune system. Hantavirus infection of these animals resulted in 
      systemic infection associated with weight loss, decreased activity, ruffled fur 
      and inflammatory infiltrates of lung tissue. Intriguingly, after infection, 
      humanized mice harbouring human leukocyte antigen (HLA) class I-restricted human 
      CD8+ T cells started to lose weight earlier (day 10) than HLA class I-negative 
      humanized mice (day 15). Moreover, in these mice the number of human platelets 
      dropped by 77 % whereas the number of murine platelets did not change, 
      illustrating how differences between rodent and human haemato-lymphoid systems 
      may contribute to disease development. To our knowledge this is the first 
      description of a humanized mouse model of hantavirus infection, and our results 
      indicate a role for human immune cells in hantaviral pathogenesis.
CI  - (c) 2015 The Authors.
FAU - Kobak, Lidija
AU  - Kobak L
AD  - Institute of Medical Virology, Helmut-Ruska-Haus, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
FAU - Raftery, Martin J
AU  - Raftery MJ
AD  - Institute of Medical Virology, Helmut-Ruska-Haus, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
FAU - Voigt, Sebastian
AU  - Voigt S
AD  - Department of Pediatric Oncology and Hematology, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
AD  - Division of Viral Infections, Robert Koch Institute, Berlin, Germany.
FAU - Kuhl, Anja A
AU  - Kuhl AA
AD  - Department of Medicine I for Gastroenterology, Infectious Disease and 
      Rheumatology/RCIS, Charite-Universitatsmedizin Berlin, Berlin, Germany.
FAU - Kilic, Ergin
AU  - Kilic E
AD  - Institute of Pathology, Charite-Universitatsmedizin Berlin, Berlin, Germany.
FAU - Kurth, Andreas
AU  - Kurth A
AD  - Centre for Biological Threats and Special Pathogens, Robert Koch Institute, 
      Berlin, Germany.
FAU - Witkowski, Peter
AU  - Witkowski P
AD  - Institute of Medical Virology, Helmut-Ruska-Haus, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
FAU - Hofmann, Jorg
AU  - Hofmann J
AD  - Division of Virology, Labor Berlin Charite-Vivantes GmbH, Berlin, Germany.
AD  - Institute of Medical Virology, Helmut-Ruska-Haus, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
FAU - Nitsche, Andreas
AU  - Nitsche A
AD  - Centre for Biological Threats and Special Pathogens, Robert Koch Institute, 
      Berlin, Germany.
FAU - Schaade, Lars
AU  - Schaade L
AD  - Centre for Biological Threats and Special Pathogens, Robert Koch Institute, 
      Berlin, Germany.
FAU - Kruger, Detlev H
AU  - Kruger DH
AD  - Institute of Medical Virology, Helmut-Ruska-Haus, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
FAU - Schonrich, Gunther
AU  - Schonrich G
AD  - Institute of Medical Virology, Helmut-Ruska-Haus, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150212
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - *Disease Models, Animal
MH  - Orthohantavirus/growth & development/pathogenicity/*physiology
MH  - Hantavirus Infections/*pathology/*virology
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Mice, SCID
MH  - Platelet Count
EDAT- 2015/02/14 06:00
MHDA- 2015/08/22 06:00
CRDT- 2015/02/14 06:00
PHST- 2014/11/07 00:00 [received]
PHST- 2015/02/04 00:00 [accepted]
PHST- 2015/02/14 06:00 [entrez]
PHST- 2015/02/14 06:00 [pubmed]
PHST- 2015/08/22 06:00 [medline]
AID - 10.1099/vir.0.000087 [doi]
PST - ppublish
SO  - J Gen Virol. 2015 Jun;96(Pt 6):1258-1263. doi: 10.1099/vir.0.000087. Epub 2015 
      Feb 12.