PMID- 25680902
OWN - NLM
STAT- MEDLINE
DCOM- 20150608
LR  - 20161125
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 46
IP  - 4
DP  - 2015 Apr
TI  - MDM2 and CDK4 expression in periosteal osteosarcoma.
PG  - 549-53
LID - S0046-8177(14)00515-2 [pii]
LID - 10.1016/j.humpath.2014.12.006 [doi]
AB  - Periosteal osteosarcoma is defined by the World Health Organization as an 
      intermediate-grade, malignant, cartilaginous, and bone-forming neoplasm arising 
      on the surface of bone. Unlike other subtypes of osteosarcoma, no data have been 
      published about mouse double minute 2 (MDM2) and cyclin-dependent kinase 4 (CDK4) 
      expression. For this reason, we evaluated the molecular and immunohistochemical 
      features of MDM2 and CDK4 in 27 cases relative to 20 patients with a diagnosis of 
      periosteal osteosarcoma, surgically treated at the Rizzoli Institute between 1981 
      and 2014. When possible, these results were compared with the MDM2 amplification 
      status as determined by fluorescence in situ hybridization (FISH). All but 1 case 
      (26/27, 96.3%) were negative for MDM2 protein using immunohistochemistry both in 
      primary and in recurrent periosteal osteosarcoma, whereas gene amplification of 
      MDM2 was not detected in any tumor analyzed (10 cases). The positive 
      immunohistochemical case shows a weak/moderate focal nuclear expression of MDM2 
      antibody in the prevalent cartilaginous component and in the spindle cells of 
      peripheral fibroblastic areas associated with osteoid production in a primary 
      periosteal osteosarcoma. CDK4 immunohistochemical expression was negative in all 
      27 cases. This retrospective analysis has demonstrated that MDM2 and CDK4 are 
      very rarely expressed in primary and recurrent periosteal osteosarcomas and 
      therefore do not appear to be molecules central to the control of cancer 
      development, growth, and progression in periosteal osteosarcoma. Therefore, when 
      compared with low-grade central and parosteal osteosarcomas, MDM2 and CDK4 
      markers cannot be used diagnostically to differentiate this subtype of 
      osteosarcoma.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Righi, Alberto
AU  - Righi A
AD  - Pathology Department, Rizzoli Institute, Bologna, Italy 40136. Electronic 
      address: alberto.righi@ior.it.
FAU - Gambarotti, Marco
AU  - Gambarotti M
AD  - Pathology Department, Rizzoli Institute, Bologna, Italy 40136.
FAU - Benini, Stefania
AU  - Benini S
AD  - Pathology Department, Rizzoli Institute, Bologna, Italy 40136.
FAU - Gamberi, Gabriella
AU  - Gamberi G
AD  - Pathology Department, Rizzoli Institute, Bologna, Italy 40136; Department of 
      Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy 40126.
FAU - Cocchi, Stefania
AU  - Cocchi S
AD  - Pathology Department, Rizzoli Institute, Bologna, Italy 40136.
FAU - Picci, Piero
AU  - Picci P
AD  - Pathology Department, Rizzoli Institute, Bologna, Italy 40136.
FAU - Bertoni, Franco
AU  - Bertoni F
AD  - Pathology Department, Rizzoli Institute, Bologna, Italy 40136; Department of 
      Pathology, Villa Erbosa Hospital, Bologna, Italy 40129.
LA  - eng
PT  - Journal Article
DEP - 20141231
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
RN  - EC 2.7.11.22 (CDK4 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 4)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Biomarkers, Tumor/*metabolism
MH  - Bone Neoplasms/*metabolism/pathology
MH  - Child
MH  - Cyclin-Dependent Kinase 4/*metabolism
MH  - Female
MH  - Gene Amplification/physiology
MH  - Humans
MH  - Male
MH  - Neoplasm Recurrence, Local/genetics
MH  - Osteosarcoma/genetics/*metabolism/pathology
MH  - Periosteum/*metabolism
MH  - Proto-Oncogene Proteins c-mdm2/*metabolism
MH  - Retrospective Studies
MH  - Young Adult
OTO - NOTNLM
OT  - Bone
OT  - CDK4
OT  - Immunohistochemistry
OT  - MDM2
OT  - Perisoteal osteosarcoma
EDAT- 2015/02/15 06:00
MHDA- 2015/06/09 06:00
CRDT- 2015/02/15 06:00
PHST- 2014/10/29 00:00 [received]
PHST- 2014/12/16 00:00 [revised]
PHST- 2014/12/17 00:00 [accepted]
PHST- 2015/02/15 06:00 [entrez]
PHST- 2015/02/15 06:00 [pubmed]
PHST- 2015/06/09 06:00 [medline]
AID - S0046-8177(14)00515-2 [pii]
AID - 10.1016/j.humpath.2014.12.006 [doi]
PST - ppublish
SO  - Hum Pathol. 2015 Apr;46(4):549-53. doi: 10.1016/j.humpath.2014.12.006. Epub 2014 
      Dec 31.