PMID- 25681161 OWN - NLM STAT- MEDLINE DCOM- 20160401 LR - 20181113 IS - 1573-6903 (Electronic) IS - 0364-3190 (Linking) VI - 40 IP - 5 DP - 2015 May TI - Acute Administration of Branched-Chain Amino Acids Increases the Pro-BDNF/Total-BDNF Ratio in the Rat Brain. PG - 885-93 LID - 10.1007/s11064-015-1541-1 [doi] AB - Maple syrup urine disease (MSUD) is caused by an inborn error in metabolism resulting from a deficiency in the branched-chain alpha-keto acid dehydrogenase complex activity. This blockage leads to accumulation of the branched-chain amino acids (BCAA) leucine, isoleucine and valine, as well as their corresponding alpha-keto acids and alpha-hydroxy acids. High levels of BCAAs are associated with neurological dysfunction and the role of pro- and mature brain-derived neurotrophic factor (BDNF) in the neurological dysfunction of MSUD is still unclear. Thus, in the present study we investigated the effect of an acute BCAA pool administration on BDNF levels and on the pro-BDNF cleavage-related proteins S100A10 and tissue plasminogen activator (tPA) in rat brains. Our results demonstrated that acute Hyper-BCAA (H-BCAA) exposure during the early postnatal period increases pro-BDNF and total-BDNF levels in the hippocampus and striatum. Moreover, tPA levels were significantly decreased, without modifications in the tPA transcript levels in the hippocampus and striatum. On the other hand, the S100A10 mRNA and S100A10 protein levels were not changed in the hippocampus and striatum. In the 30-day-old rats, we observed increased pro-BDNF, total-BDNF and tPA levels only in the striatum, whereas the tPA and S100A10 mRNA expression and the immunocontent of S100A10 were not altered. In conclusion, we demonstrated that acute H-BCAA administration increases the pro-BDNF/total-BDNF ratio and decreases the tPA levels in animals, suggesting that the BCAA effect may depend, at least in part, on changes in BDNF post-translational processing. FAU - Scaini, Giselli AU - Scaini G AD - Laboratorio de Bioenergetica e Nucleo de Excelencia em Neurociencias Aplicadas de Santa Catarina (NENASC), Programa de Pos-Graduacao em Ciencias da Saude, Universidade do Extremo Sul Catarinense, Av. Universitaria, 1105, Criciuma, SC, 88806-000, Brazil. FAU - Morais, Meline O S AU - Morais MO FAU - Furlanetto, Camila B AU - Furlanetto CB FAU - Kist, Luiza W AU - Kist LW FAU - Pereira, Talita C B AU - Pereira TC FAU - Schuck, Patricia F AU - Schuck PF FAU - Ferreira, Gustavo C AU - Ferreira GC FAU - Pasquali, Matheus A B AU - Pasquali MA FAU - Gelain, Daniel P AU - Gelain DP FAU - Moreira, Jose Claudio F AU - Moreira JC FAU - Bogo, Mauricio R AU - Bogo MR FAU - Streck, Emilio L AU - Streck EL LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150214 PL - United States TA - Neurochem Res JT - Neurochemical research JID - 7613461 RN - 0 (Amino Acids, Branched-Chain) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Protein Precursors) RN - 0 (brain-derived neurotrophic factor precursor) SB - IM MH - Amino Acids, Branched-Chain/*administration & dosage MH - Animals MH - Brain-Derived Neurotrophic Factor/*biosynthesis MH - Hippocampus/*drug effects/*metabolism MH - Injections, Subcutaneous MH - Male MH - Neostriatum/*drug effects/*metabolism MH - Protein Precursors/*biosynthesis MH - Rats MH - Rats, Wistar EDAT- 2015/02/15 06:00 MHDA- 2016/04/02 06:00 CRDT- 2015/02/15 06:00 PHST- 2014/10/20 00:00 [received] PHST- 2015/02/10 00:00 [accepted] PHST- 2015/01/22 00:00 [revised] PHST- 2015/02/15 06:00 [entrez] PHST- 2015/02/15 06:00 [pubmed] PHST- 2016/04/02 06:00 [medline] AID - 10.1007/s11064-015-1541-1 [doi] PST - ppublish SO - Neurochem Res. 2015 May;40(5):885-93. doi: 10.1007/s11064-015-1541-1. Epub 2015 Feb 14.