PMID- 25681629
OWN - NLM
STAT- MEDLINE
DCOM- 20151224
LR  - 20150318
IS  - 1879-0720 (Electronic)
IS  - 0928-0987 (Linking)
VI  - 71
DP  - 2015 Apr 25
TI  - Silica nanoparticles: preparation, characterization and in vitro/in vivo 
      biodistribution studies.
PG  - 46-55
LID - S0928-0987(15)00052-4 [pii]
LID - 10.1016/j.ejps.2015.02.002 [doi]
AB  - BACKGROUND: The current progress in pharmaceutical nanotechnology field has been 
      exploited in the design of functionalized radiolabelled nanoparticles that are 
      able to deliver radionuclides in a selective manner to improve the outcome of 
      diagnosis and treatment. Silica nanoparticles (SNPs) have been widely developed 
      for biomedical applications due to their high versatility, excellent functional 
      properties and low cost production, with the possibility to control different 
      topological parameters relevant for multidisciplinary applications. PURPOSE: The 
      aim of the present study was to characterize and evaluate both in vitro, by 
      microscopy techniques, and in vivo, by scintigraphic imaging, the biodistribution 
      of silica nanostructures derivatives (Cy5.5 conjugated SNPs and (99m)Tc 
      radiolabelled SNPs) to be applied as radiotracers in biomedicine. METHODS: SNPs 
      were synthesized by hydrolysis and condensation of silicon alkoxides, followed by 
      surface functionalization with amino groups available for fluorescent dye and 
      radiolabelling possibility. RESULTS: Our data showed the particles size 
      distribution (200-350 nm), the surface charge (negative for bare and fluorescent 
      SNPs and positive for amino SNPs), polydispersity index (broad distribution), the 
      qualitative composition and the toxicity assessments (safe material) that made 
      the obtained SNPs candidates for in vitro/in vivo studies. A high uptake of 
      fluorescent SNPs in all the investigated organs was evidenced by confocal 
      microscopy. The (99m)Tc radiolabelled SNPs biodistribution was quantified in the 
      range of 12-100% counts/g organ using the scintigraphic images. CONCLUSIONS: The 
      obtained results reveal improved properties, namely, reduced toxicity with a low 
      level of side effects, an improved biodistribution, high labelling efficiency and 
      stability of the radiolabelled SNPs with potential to be applied in biomedical 
      science, particularly in nuclear medicine as a radiotracer.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Tamba, B I
AU  - Tamba BI
AD  - Centre for the Study and Therapy of Pain, "Gr. T. Popa" University of Medicine 
      and Pharmacy, Iasi, Romania.
FAU - Dondas, A
AU  - Dondas A
AD  - Centre for the Study and Therapy of Pain, "Gr. T. Popa" University of Medicine 
      and Pharmacy, Iasi, Romania.
FAU - Leon, M
AU  - Leon M
AD  - Centre for the Study and Therapy of Pain, "Gr. T. Popa" University of Medicine 
      and Pharmacy, Iasi, Romania.
FAU - Neagu, A N
AU  - Neagu AN
AD  - Laboratory of Animal Histology, Faculty of Biology, "Alexandru Ioan Cuza" 
      University of Iasi, Romania.
FAU - Dodi, G
AU  - Dodi G
AD  - Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" 
      Technical University of Iasi, Romania; SCIENT - Research Centre for Instrumental 
      Analysis, Bucharest, Romania.
FAU - Stefanescu, C
AU  - Stefanescu C
AD  - Department of Biophysics and Medical Physics, "Gr. T. Popa" University of 
      Medicine and Pharmacy, 700115 Iasi, Romania. Electronic address: 
      cipriana.stefanescu@yahoo.com.
FAU - Tijani, A
AU  - Tijani A
AD  - FHNW, School of Life Sciences, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150211
PL  - Netherlands
TA  - Eur J Pharm Sci
JT  - European journal of pharmaceutical sciences : official journal of the European 
      Federation for Pharmaceutical Sciences
JID - 9317982
RN  - 0 (CY5.5 cyanine dye)
RN  - 0 (Carbocyanines)
RN  - 0 (Fluorescent Dyes)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Carbocyanines/pharmacokinetics
MH  - Drug Compounding
MH  - Fluorescent Dyes/pharmacokinetics
MH  - Guinea Pigs
MH  - Male
MH  - Mice
MH  - *Nanoparticles/toxicity
MH  - Particle Size
MH  - Silicon Dioxide/*pharmacokinetics/toxicity
MH  - Surface Properties
MH  - Tissue Distribution
OTO - NOTNLM
OT  - Biodistribution
OT  - Fluorescent
OT  - Microscopy
OT  - Scintigraphy
OT  - Silica nanoparticles
EDAT- 2015/02/15 06:00
MHDA- 2015/12/25 06:00
CRDT- 2015/02/15 06:00
PHST- 2014/08/20 00:00 [received]
PHST- 2015/01/09 00:00 [revised]
PHST- 2015/02/02 00:00 [accepted]
PHST- 2015/02/15 06:00 [entrez]
PHST- 2015/02/15 06:00 [pubmed]
PHST- 2015/12/25 06:00 [medline]
AID - S0928-0987(15)00052-4 [pii]
AID - 10.1016/j.ejps.2015.02.002 [doi]
PST - ppublish
SO  - Eur J Pharm Sci. 2015 Apr 25;71:46-55. doi: 10.1016/j.ejps.2015.02.002. Epub 2015 
      Feb 11.