PMID- 25682741
OWN - NLM
STAT- MEDLINE
DCOM- 20150817
LR  - 20181202
IS  - 1930-739X (Electronic)
IS  - 1930-7381 (Linking)
VI  - 23
IP  - 3
DP  - 2015 Mar
TI  - DBC1 is involved in adipocyte inflammation and is a possible marker of human 
      adipose tissue senescence.
PG  - 519-22
LID - 10.1002/oby.20999 [doi]
AB  - OBJECTIVE: To investigate the possible role of deleted in breast cancer 1 (DBC1) 
      in adipocyte and adipose tissue inflammation. METHODS: In vitro knockdown 
      experiments using shRNA-lentiviral particles were performed to investigate the 
      effect of DBC1 on adipocyte inflammation, sirtuin 1 (Sirt1) activity, and the 
      AMPK pathway. The relationship between DBC1 and inflammation in human adipose 
      tissue also was examined in two independent cohorts. RESULTS: Dbc1 knockdown (KD) 
      led to a significant reduction in the expression of inflammatory genes (Tnf, Il6, 
      Stamp2, Lbp, and Mcp1) and (pSer536) NF-kappaB (p65)/NF-kappaB (p65) ratio in fully 
      differentiated adipocytes. Of note, Dbc1 KD increased Sirt1 and AMPK activity in 
      the early stage of adipocyte differentiation. In morbidly obese participants, 
      DBC1 was positively correlated to TNF and senescence (TP53 and BAX) gene 
      expression markers in both subcutaneous and visceral adipose tissues. 
      Multivariate regression analysis revealed that senescence-related gene markers 
      were the best predictors of adipose tissue DBC1 mRNA levels. CONCLUSIONS: DBC1 
      induced the expression of nuclear factor kappa B (NF-kappaB)-regulated inflammatory 
      cytokines in fully differentiated 3T3-L1 adipocytes, possibly through the 
      inhibition of Sirt1 activity, being significantly associated with human adipose 
      tissue senescence in morbidly obese subjects.
CI  - (c) 2015 The Obesity Society.
FAU - Moreno-Navarrete, Jose Maria
AU  - Moreno-Navarrete JM
AD  - Department of Diabetes, Endocrinology and Nutrition, Institut d'Investigacio 
      Biomedica de Girona (IdIBGi), CIBEROBN (CB06/03/010) and Instituto de Salud 
      Carlos III (ISCIII), Girona, Spain.
FAU - Moreno, Maria
AU  - Moreno M
FAU - Vidal, Marta
AU  - Vidal M
FAU - Ortega, Francisco
AU  - Ortega F
FAU - Ricart, Wifredo
AU  - Ricart W
FAU - Fernandez-Real, Jose Manuel
AU  - Fernandez-Real JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150213
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biomarkers)
RN  - 0 (CCAR2 protein, human)
RN  - 0 (KIAA1967 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Adaptor Proteins, Signal Transducing/genetics/*metabolism
MH  - Adipocytes/metabolism
MH  - Adipose Tissue/*metabolism
MH  - Animals
MH  - Biomarkers/*metabolism
MH  - Cellular Senescence
MH  - Cohort Studies
MH  - Gene Expression
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Mice
MH  - Obesity, Morbid/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Sirtuin 1/metabolism
EDAT- 2015/02/17 06:00
MHDA- 2015/08/19 06:00
CRDT- 2015/02/17 06:00
PHST- 2014/06/11 00:00 [received]
PHST- 2014/11/03 00:00 [revised]
PHST- 2014/11/14 00:00 [accepted]
PHST- 2015/02/17 06:00 [entrez]
PHST- 2015/02/17 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
AID - 10.1002/oby.20999 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2015 Mar;23(3):519-22. doi: 10.1002/oby.20999. Epub 2015 
      Feb 13.