PMID- 25683113
OWN - NLM
STAT- MEDLINE
DCOM- 20151228
LR  - 20210119
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Print)
IS  - 0034-5288 (Linking)
VI  - 99
DP  - 2015 Apr
TI  - Small interfering RNA targeting nonstructural protein1 alpha (nsp1alpha) of porcine 
      reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of 
      PRRSV in MARC-145 cells.
PG  - 215-7
LID - S0034-5288(15)00040-5 [pii]
LID - 10.1016/j.rvsc.2015.01.015 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most 
      economically devastating and pandemic diseases of swine, which is poorly 
      controlled by current methods. The inhibition of specific genes by small 
      interfering RNA (siRNA) has been proven to be a potential therapeutic strategy 
      against viral infection. Previous studies have indicated that the nonstructural 
      protein 1alpha (nsp1alpha) of PRRSV may take an important role in virulence of PRRSV. The 
      present work was involved to explore the effect of siRNA targeting nsp1alpha on the 
      replication of PRRSV in MARC-145 cells, and the results showed that 
      over-expression of nsp1alpha enhanced the replication of PRRSV and that siRNAs 
      specifically targeting nsp1alpha significantly inhibited the replication of PRRSV in 
      MARC-145 cells. In conclusion, this work indicated that nsp1alpha may be a viral 
      pathogenicity factor of PRRSV and that siRNAs specifically targeting nsp1alpha may be 
      a new strategy to control PRRSV in the future.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Shi, Xibao
AU  - Shi X
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China; 
      College of Veterinary Medicine and Animal Science, Henan Agricultural University, 
      Zhengzhou, Henan 450002, China; Key Laboratory of Animal Immunology of the 
      Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, 
      Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
FAU - Chang, Yongzhe
AU  - Chang Y
AD  - College of Veterinary Medicine and Animal Science, Henan Agricultural University, 
      Zhengzhou, Henan 450002, China; Key Laboratory of Animal Immunology of the 
      Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, 
      Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
FAU - Zhang, Xiaozhuan
AU  - Zhang X
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China; 
      College of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 
      453007, China.
FAU - Wang, Li
AU  - Wang L
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, Henan 450002, China.
FAU - Li, Chunxi
AU  - Li C
AD  - College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
FAU - Jiang, Kai
AU  - Jiang K
AD  - College of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 
      453007, China.
FAU - Chen, Jing
AU  - Chen J
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, Henan 450002, China.
FAU - Wang, Chao
AU  - Wang C
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, Henan 450002, China.
FAU - Deng, Ruiguang
AU  - Deng R
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, Henan 450002, China.
FAU - Fan, Jianming
AU  - Fan J
AD  - The Laboratory of Toxicology, College of Public Health, Zhengzhou University, 
      Zhengzhou 450001, China.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - College of Veterinary Medicine and Animal Science, Henan Agricultural University, 
      Zhengzhou, Henan 450002, China; Key Laboratory of Animal Immunology of the 
      Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, 
      Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      Electronic address: zhanggaiping2003@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150130
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Haplorhini
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - RNA, Small Interfering/*genetics/metabolism
MH  - Viral Nonstructural Proteins/*genetics/metabolism
MH  - *Virus Replication
PMC - PMC7111734
OTO - NOTNLM
OT  - PRRSV
OT  - Small interfering RNA
OT  - nsp1alpha
EDAT- 2015/02/17 06:00
MHDA- 2015/12/29 06:00
PMCR- 2015/01/30
CRDT- 2015/02/17 06:00
PHST- 2014/08/15 00:00 [received]
PHST- 2015/01/23 00:00 [revised]
PHST- 2015/01/24 00:00 [accepted]
PHST- 2015/02/17 06:00 [entrez]
PHST- 2015/02/17 06:00 [pubmed]
PHST- 2015/12/29 06:00 [medline]
PHST- 2015/01/30 00:00 [pmc-release]
AID - S0034-5288(15)00040-5 [pii]
AID - 10.1016/j.rvsc.2015.01.015 [doi]
PST - ppublish
SO  - Res Vet Sci. 2015 Apr;99:215-7. doi: 10.1016/j.rvsc.2015.01.015. Epub 2015 Jan 
      30.