PMID- 25683727
OWN - NLM
STAT- MEDLINE
DCOM- 20160324
LR  - 20220321
IS  - 1399-0012 (Electronic)
IS  - 0902-0063 (Linking)
VI  - 29
IP  - 5
DP  - 2015 May
TI  - Clinical impact of preformed donor-specific denatured class I HLA antibodies 
      after kidney transplantation.
PG  - 393-402
LID - 10.1111/ctr.12529 [doi]
AB  - Class I single-antigen flow beads (SAFB) carry native and denatured human 
      leukocyte antigen (HLA) molecules. Using a cohort of 179 class I HLA-sensitized 
      kidney recipients, we described incidence and clinical relevance of preformed 
      denatured HLA donor-specific antibodies (DSA) using two different assays: an 
      acid-treated SAFB assay (anti-dHLA DSA) and the iBeads assays (SAFB+/iBeads- 
      DSA). Eighty-five class I DSA were found in 67 patients (median mean fluorescence 
      intensity [MFI] of 1729 [range 520-13 882]). Anti-dHLA and SAFB+/iBeads- DSA 
      represented 11% and 18% of class I DSA and were mainly low MFI DSA (500-1000 
      MFI). Concordance between these two assays was good (90%). None of the patients 
      with only class I anti-dHLA DSA or only SAFB+/iBeads- DSA developed acute 
      clinical antibody-mediated rejection in the first-year post-transplantation, and 
      their five-yr death-censored graft survival was similar to that of patients 
      without DSA. Moreover, all these patients displayed a negative current T-cell 
      flow cytometry cross-match. Therefore, both anti-dHLA DSA and SAFB+/iBeads- DSA 
      appear irrelevant, which could explain the good outcome observed in some patients 
      with preformed class I DSA.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Visentin, Jonathan
AU  - Visentin J
AD  - Laboratoire d'Immunologie et Immunogenetique, Hopital Pellegrin, CHU de Bordeaux, 
      Bordeaux, France; UMR CNRS 5164, Universite de Bordeaux, Bordeaux, France.
FAU - Marroc, Melanie
AU  - Marroc M
FAU - Guidicelli, Gwendaline
AU  - Guidicelli G
FAU - Bachelet, Thomas
AU  - Bachelet T
FAU - Nong, Thoa
AU  - Nong T
FAU - Moreau, Jean-Francois
AU  - Moreau JF
FAU - Lee, Jar-How
AU  - Lee JH
FAU - Merville, Pierre
AU  - Merville P
FAU - Couzi, Lionel
AU  - Couzi L
FAU - Taupin, Jean-Luc
AU  - Taupin JL
LA  - eng
PT  - Journal Article
DEP - 20150313
PL  - Denmark
TA  - Clin Transplant
JT  - Clinical transplantation
JID - 8710240
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Antibody Specificity
MH  - Female
MH  - Follow-Up Studies
MH  - Glomerular Filtration Rate
MH  - Graft Survival/*immunology
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Isoantibodies/blood/*immunology
MH  - Kidney Failure, Chronic/*immunology/surgery
MH  - Kidney Function Tests
MH  - *Kidney Transplantation
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Protein Denaturation
MH  - Retrospective Studies
MH  - Risk Factors
MH  - Tissue Donors
OTO - NOTNLM
OT  - antibody-mediated rejection
OT  - denatured class I HLA Antibodies
OT  - donor-specific antibodies
OT  - human leukocyte antigen
OT  - kidney transplantation
EDAT- 2015/02/17 06:00
MHDA- 2016/03/25 06:00
CRDT- 2015/02/17 06:00
PHST- 2015/02/09 00:00 [accepted]
PHST- 2015/02/17 06:00 [entrez]
PHST- 2015/02/17 06:00 [pubmed]
PHST- 2016/03/25 06:00 [medline]
AID - 10.1111/ctr.12529 [doi]
PST - ppublish
SO  - Clin Transplant. 2015 May;29(5):393-402. doi: 10.1111/ctr.12529. Epub 2015 Mar 
      13.