PMID- 25686501
OWN - NLM
STAT- MEDLINE
DCOM- 20150526
LR  - 20171116
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 458
IP  - 4
DP  - 2015 Mar 20
TI  - The activation of peroxisome proliferator-activated receptor gamma is regulated by 
      Kruppel-like transcription factors 6 & 9 under steatotic conditions.
PG  - 751-6
LID - S0006-291X(15)00192-8 [pii]
LID - 10.1016/j.bbrc.2015.01.145 [doi]
AB  - Liver steatosis is characterised by lipid droplet deposition in hepatocytes that 
      can leads to an inflammatory and fibrotic phenotype. Peroxisome 
      proliferator-activated receptors (PPARs) play key roles in energetic homeostasis 
      by regulating lipid metabolism in hepatic tissue. In adipose tissue PPARgamma 
      regulates the adipocyte differentiation by promoting the expression of 
      lipid-associated genes. Within the liver PPARgamma is up-regulated under steatotic 
      conditions; however, which transcription factors participate in its expression is 
      not completely understood. Kruppel-like transcription factors (KLFs) regulate 
      various cellular mechanisms, such as cell proliferation and differentiation. KLFs 
      are key components of adipogenesis by regulating the expression of PPARgamma and 
      other proteins such as the C-terminal enhancer binding protein (C/EBP). Here, we 
      demonstrate that the transcript levels of Klf6, Klf9 and Ppargamma are increased in 
      response to a steatotic insult in vitro. Chromatin immunoprecipitation (ChIp) 
      experiments showed that klf6 and klf9 are actively recruited to the Ppargamma 
      promoter region under these conditions. Accordingly, the loss-of-function 
      experiments reduced cytoplasmic triglyceride accumulation. Here, we demonstrated 
      that KLF6 and KLF9 proteins directly regulate PPARgamma expression under steatotic 
      conditions.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Escalona-Nandez, Ivonne
AU  - Escalona-Nandez I
AD  - Departamento de Gastroenterologia, Instituto Nacional de Ciencias Medicas y 
      Nutricion Salvador Zubiran, Vasco de Quiroga 15 Seccion XVI, Tlalpan, 14000, 
      Mexico, D.F., Mexico.
FAU - Guerrero-Escalera, Dafne
AU  - Guerrero-Escalera D
AD  - Departamento de Gastroenterologia, Instituto Nacional de Ciencias Medicas y 
      Nutricion Salvador Zubiran, Vasco de Quiroga 15 Seccion XVI, Tlalpan, 14000, 
      Mexico, D.F., Mexico.
FAU - Estanes-Hernandez, Alma
AU  - Estanes-Hernandez A
AD  - Departamento de Gastroenterologia, Instituto Nacional de Ciencias Medicas y 
      Nutricion Salvador Zubiran, Vasco de Quiroga 15 Seccion XVI, Tlalpan, 14000, 
      Mexico, D.F., Mexico.
FAU - Ortiz-Ortega, Victor
AU  - Ortiz-Ortega V
AD  - Departamento de Fisiologia de la Nutricion, Instituto Nacional de Ciencias 
      Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga 15 Seccion XVI, Tlalpan, 
      14000, Mexico, D.F., Mexico.
FAU - Tovar, Armando R
AU  - Tovar AR
AD  - Departamento de Fisiologia de la Nutricion, Instituto Nacional de Ciencias 
      Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga 15 Seccion XVI, Tlalpan, 
      14000, Mexico, D.F., Mexico.
FAU - Perez-Monter, Carlos
AU  - Perez-Monter C
AD  - Departamento de Gastroenterologia, Instituto Nacional de Ciencias Medicas y 
      Nutricion Salvador Zubiran, Vasco de Quiroga 15 Seccion XVI, Tlalpan, 14000, 
      Mexico, D.F., Mexico. Electronic address: carlos.perezm@incmnsz.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150214
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (KLF6 protein, human)
RN  - 0 (KLF9 protein, human)
RN  - 0 (Kruppel-Like Factor 6)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (PPAR gamma)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Triglycerides)
RN  - 2V16EO95H1 (Palmitic Acid)
SB  - IM
MH  - Fatty Liver/genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Hep G2 Cells
MH  - Humans
MH  - Kruppel-Like Factor 6
MH  - Kruppel-Like Transcription Factors/genetics/*metabolism
MH  - PPAR gamma/*genetics/metabolism
MH  - Palmitic Acid/*metabolism
MH  - Promoter Regions, Genetic
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Transcriptional Activation
MH  - Triglycerides/metabolism
OTO - NOTNLM
OT  - Gene expression
OT  - KLF6
OT  - KLF9
OT  - PPARgamma
OT  - Steatosis
OT  - sh-RNA
EDAT- 2015/02/18 06:00
MHDA- 2015/05/27 06:00
CRDT- 2015/02/18 06:00
PHST- 2015/01/26 00:00 [received]
PHST- 2015/01/29 00:00 [accepted]
PHST- 2015/02/18 06:00 [entrez]
PHST- 2015/02/18 06:00 [pubmed]
PHST- 2015/05/27 06:00 [medline]
AID - S0006-291X(15)00192-8 [pii]
AID - 10.1016/j.bbrc.2015.01.145 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Mar 20;458(4):751-6. doi: 
      10.1016/j.bbrc.2015.01.145. Epub 2015 Feb 14.