PMID- 25687641
OWN - NLM
STAT- MEDLINE
DCOM- 20160418
LR  - 20181113
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 38
IP  - 4
DP  - 2015 Aug
TI  - Triptolide Protects Against Ischemic Stroke in Rats.
PG  - 1617-23
LID - 10.1007/s10753-015-0137-x [doi]
AB  - Inflammatory response plays an important role in the pathogenesis of ischemic 
      stroke and anti-inflammatory agents may provide a choice of treatment. Triptolide 
      is reported to be anti-inflammatory. In this study, we investigated the effects 
      of triptolide on cultured neuronal cell line in vitro and experimental ischemic 
      stroke in vivo. Oxygen-glucose deprivation (OGD) and tumor necrosis factor-alpha 
      (TNF-alpha) stimulated SH-SY5Y cells were incubated with triptolide. In vivo, rats 
      were subjected to middle cerebral artery occlusion (MCAO) for 1 h, followed by 
      reperfusion for 23 h. Results of this study showed that triptolide treatment 
      reduced the OGD-induced cytotoxicity and apoptosis and blocked TNF-alpha-induced 
      activation of NF-kappaB and p38MAPK in SH-SY5Y cells. Intraperitoneal injection of 
      triptolide showed significant neuroprotective actions in stroke rats. Triptolide 
      attenuated neurological deficit, brain infarct volume, and brain water content, 
      and inhibited activation of NF-kappaB and p38MAPK. These data show that triptolide 
      protects rats against ischemic cerebral injury via inhibiting NF-kappaB and p38MAPK 
      signaling pathways.
FAU - Hao, Maolin
AU  - Hao M
AD  - Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong 
      University, #4 Duanxing West Road, Jinan, 250022, Shandong, China, 
      hao_maolin@163.com.
FAU - Li, Xianghua
AU  - Li X
FAU - Feng, Jianli
AU  - Feng J
FAU - Pan, Ning
AU  - Pan N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Diterpenes)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Phenanthrenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 19ALD1S53J (triptolide)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/metabolism/*prevention & control
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects/physiology
MH  - Diterpenes/pharmacology/*therapeutic use
MH  - Dose-Response Relationship, Drug
MH  - Epoxy Compounds/pharmacology/therapeutic use
MH  - Humans
MH  - Male
MH  - Neuroprotective Agents/pharmacology/*therapeutic use
MH  - Phenanthrenes/pharmacology/*therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stroke/metabolism/*prevention & control
MH  - Tumor Necrosis Factor-alpha/antagonists & inhibitors/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
EDAT- 2015/02/18 06:00
MHDA- 2016/04/19 06:00
CRDT- 2015/02/18 06:00
PHST- 2015/02/18 06:00 [entrez]
PHST- 2015/02/18 06:00 [pubmed]
PHST- 2016/04/19 06:00 [medline]
AID - 10.1007/s10753-015-0137-x [doi]
PST - ppublish
SO  - Inflammation. 2015 Aug;38(4):1617-23. doi: 10.1007/s10753-015-0137-x.