PMID- 25689487
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160115
LR  - 20220330
IS  - 1386-6346 (Print)
IS  - 1872-034X (Electronic)
IS  - 1386-6346 (Linking)
VI  - 45
IP  - 13
DP  - 2015 Dec
TI  - High incidence of the hepatitis C virus recombinant 2k/1b in Georgia: 
      Recommendations for testing and treatment.
PG  - 1292-8
LID - 10.1111/hepr.12505 [doi]
AB  - AIM: The first hepatitis C virus (HCV) recombinant, RF2k/1b, was initially 
      described from Russia and has since then been identified from patients in 
      Ireland, Estonia, Uzbekistan and Cyprus. Many of these patients originated from 
      Georgia; however, there is no information on its prevalence in Georgia or its 
      susceptibility to antiviral treatment. METHODS: We retrospectively sequenced the 
      non-structural region 5B (NS5B) of the HCV genome in samples from 72 Georgian 
      patients, 36 of whom had been treated with pegylated interferon and ribavirin. 
      RESULTS: The HCV genotype was determined using the Versant HCV Genotype v2 kit. 
      Based on this typing, 32 patients (44.4%) were infected with genotype 1, 21 
      (29.1%) genotype 2 and 19 (26.3%) genotype 3. Partial NS5B of these strains was 
      sequenced and analyzed for type, with concordant genotype results for all type 1 
      and 3 strains. Discrepant results were observed for genotyped 2 strains, with 16 
      (76%) having NS5B of subtype 1b. On phylogenetic analysis, 15 NS5B sequences of 
      these strains were found in a clade formed by recombinant RF2k/1b strains. The 
      remaining discordant sequence was found within a clade formed by 1b strains. 
      CONCLUSION: Our findings show that the RF2k/1b recombinant strain is common among 
      Georgian patients previously assumed to be infected with genotype 2. Because 
      genotyping is mainly performed to decide treatment strategies, there is a need to 
      determine the genotype by analysis of at least two genomic regions in strains 
      from Georgian patients considered infected with genotype 2 based on standard HCV 
      genotyping methods.
CI  - (c) 2015 The Japan Society of Hepatology.
FAU - Karchava, Marine
AU  - Karchava M
AD  - Infectious Diseases, AIDS and Clinical Immunology Research Center.
FAU - Waldenstrom, Jesper
AU  - Waldenstrom J
AD  - Department of Infectious Diseases, Institute of Biomedicine, University of 
      Gothenburg, Gothenburg, Sweden.
FAU - Parker, Monica
AU  - Parker M
AD  - David Axelrod Institute, Wadsworth Center, Albany, New York, USA.
FAU - Hallack, Renee
AU  - Hallack R
AD  - David Axelrod Institute, Wadsworth Center, Albany, New York, USA.
FAU - Sharvadze, Lali
AU  - Sharvadze L
AD  - Infectious Diseases, AIDS and Clinical Immunology Research Center.
AD  - Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia.
FAU - Gatserelia, Lana
AU  - Gatserelia L
AD  - Infectious Diseases, AIDS and Clinical Immunology Research Center.
FAU - Chkhartishvili, Nikoloz
AU  - Chkhartishvili N
AD  - Infectious Diseases, AIDS and Clinical Immunology Research Center.
FAU - Dvali, Natia
AU  - Dvali N
AD  - Infectious Diseases, AIDS and Clinical Immunology Research Center.
FAU - Dzigua, Lela
AU  - Dzigua L
AD  - Infectious Diseases, AIDS and Clinical Immunology Research Center.
FAU - Dolmazashvili, Ekaterine
AU  - Dolmazashvili E
AD  - Infectious Diseases, AIDS and Clinical Immunology Research Center.
FAU - Norder, Helene
AU  - Norder H
AD  - Department of Infectious Diseases, Institute of Biomedicine, University of 
      Gothenburg, Gothenburg, Sweden.
FAU - Tsertsvadze, Tengiz
AU  - Tsertsvadze T
AD  - Infectious Diseases, AIDS and Clinical Immunology Research Center.
AD  - Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia.
LA  - eng
GR  - D43 TW000233/TW/FIC NIH HHS/United States
GR  - D43 TW007384/TW/FIC NIH HHS/United States
PT  - Journal Article
DEP - 20150324
PL  - Netherlands
TA  - Hepatol Res
JT  - Hepatology research : the official journal of the Japan Society of Hepatology
JID - 9711801
PMC - PMC4787595
MID - NIHMS764453
OTO - NOTNLM
OT  - hepatitis C virus sequence
OT  - hybrid virus
OT  - interferon treatment
OT  - phylogenetic analysis
EDAT- 2015/02/18 06:00
MHDA- 2015/02/18 06:01
PMCR- 2016/12/01
CRDT- 2015/02/18 06:00
PHST- 2014/12/23 00:00 [received]
PHST- 2015/01/23 00:00 [revised]
PHST- 2015/02/10 00:00 [accepted]
PHST- 2015/02/18 06:00 [entrez]
PHST- 2015/02/18 06:00 [pubmed]
PHST- 2015/02/18 06:01 [medline]
PHST- 2016/12/01 00:00 [pmc-release]
AID - 10.1111/hepr.12505 [doi]
PST - ppublish
SO  - Hepatol Res. 2015 Dec;45(13):1292-8. doi: 10.1111/hepr.12505. Epub 2015 Mar 24.