PMID- 25698877
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150220
LR  - 20240322
IS  - 1176-9351 (Print)
IS  - 1176-9351 (Electronic)
IS  - 1176-9351 (Linking)
VI  - 14
IP  - Suppl 3
DP  - 2015
TI  - Regulation of epithelial-mesenchymal transition in breast cancer cells by cell 
      contact and adhesion.
PG  - 1-13
LID - 10.4137/CIN.S18965 [doi]
AB  - Epithelial-mesenchymal transition (EMT) is a physiological program that is 
      activated during cancer cell invasion and metastasis. We show here that 
      EMT-related processes are linked to a broad and conserved program of 
      transcriptional alterations that are influenced by cell contact and adhesion. 
      Using cultured human breast cancer and mouse mammary epithelial cells, we find 
      that reduced cell density, conditions under which cell contact is reduced, leads 
      to reduced expression of genes associated with mammary epithelial cell 
      differentiation and increased expression of genes associated with breast cancer. 
      We further find that treatment of cells with matrix metalloproteinase-3 (MMP-3), 
      an inducer of EMT, interrupts a defined subset of cell contact-regulated genes, 
      including genes encoding a variety of RNA splicing proteins known to regulate the 
      expression of Rac1b, an activated splice isoform of Rac1 known to be a key 
      mediator of MMP-3-induced EMT in breast, lung, and pancreas. These results 
      provide new insights into how MMPs act in cancer progression and how loss of 
      cell-cell interactions is a key step in the earliest stages of cancer 
      development.
FAU - Cichon, Magdalena A
AU  - Cichon MA
AD  - Department of Cancer Biology, Mayo Clinic Cancer Center, Jacksonville, FL USA.
FAU - Nelson, Celeste M
AU  - Nelson CM
AD  - Department of Chemical and Biological Engineering, Princeton University, 
      Princeton, NJ, USA. ; Department of Molecular Biology, Princeton University, 
      Princeton, NJ, USA.
FAU - Radisky, Derek C
AU  - Radisky DC
AD  - Department of Cancer Biology, Mayo Clinic Cancer Center, Jacksonville, FL USA.
LA  - eng
GR  - R21 HL110335/HL/NHLBI NIH HHS/United States
GR  - R21 HL118532/HL/NHLBI NIH HHS/United States
GR  - R01 GM083997/GM/NIGMS NIH HHS/United States
GR  - R01 HL120142/HL/NHLBI NIH HHS/United States
GR  - P50 CA116201/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20150209
PL  - United States
TA  - Cancer Inform
JT  - Cancer informatics
JID - 101258149
PMC - PMC4325704
OTO - NOTNLM
OT  - breast cancer
OT  - cell contact
OT  - epithelial-mesenchymal transition
OT  - extracellular matrix
OT  - mammary epithelial cells
OT  - matrix metalloproteinases
EDAT- 2015/02/24 06:00
MHDA- 2015/02/24 06:01
PMCR- 2015/02/09
CRDT- 2015/02/21 06:00
PHST- 2014/11/25 00:00 [received]
PHST- 2014/12/29 00:00 [revised]
PHST- 2015/01/04 00:00 [accepted]
PHST- 2015/02/21 06:00 [entrez]
PHST- 2015/02/24 06:00 [pubmed]
PHST- 2015/02/24 06:01 [medline]
PHST- 2015/02/09 00:00 [pmc-release]
AID - cin-suppl.3-2015-001 [pii]
AID - 10.4137/CIN.S18965 [doi]
PST - epublish
SO  - Cancer Inform. 2015 Feb 9;14(Suppl 3):1-13. doi: 10.4137/CIN.S18965. eCollection 
      2015.