PMID- 25701211
OWN - NLM
STAT- MEDLINE
DCOM- 20150615
LR  - 20150413
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 160
IP  - 5
DP  - 2015 May
TI  - Novel rat models to study primary genital herpes simplex virus-2 infection.
PG  - 1153-61
LID - 10.1007/s00705-015-2365-7 [doi]
AB  - In this study we describe that six rat models (SD, WIST, LEW, BN, F344 and DA) 
      are susceptible to intravaginal herpes simplex virus-2 (HSV-2) infection after 
      pre-treatment with progesterone. At a virus dose of 5 x 10(6) PFU of HSV-2, all 
      rat models were infected presenting anti-HSV-2 antibodies, infectious virus in 
      vaginal washes, and HSV-2 DNA genome copies in lumbosacral dorsal root ganglia 
      and the spinal cord. Most of the LEW, BN, F344, and DA rats succumbed in systemic 
      progressive symptoms at day 8-14 post infection, but presented no or mild genital 
      inflammation while SD and WIST rats were mostly infected asymptomatically. 
      Infected SD rats did not reactivate HSV-2 spontaneously or after cortisone 
      treatment. In an HSV-2 virus dose reduction study, F344 rats were shown to be 
      most susceptible. We also investigated whether an attenuated HSV-1 strain 
      (KOS321) given intravaginally, could protect from a subsequent HSV-2 infection. 
      All LEW, BN, and F344 rats survived a primary HSV-1 infection and no neuronal 
      infection was established. In BN and F344 rats, anti-HSV-1 antibodies were 
      readily detected while LEW rats were seronegative. In contrast to naive LEW, BN, 
      and F344 rats where only 3 of 18 animals survived 5 x 10(6) PFU of HSV-2, 23 of 
      25 previously HSV-1 infected rats survived a challenge with HSV-2. The described 
      models provide a new approach to investigate protective effects of anti-viral 
      microbicides and vaccine candidates, as well as to study asymptomatic primary 
      genital HSV-2 infection.
FAU - Onnheim, Karin
AU  - Onnheim K
AD  - Section of Virology, Department of Infectious Medicine, Institute of Biomedicine, 
      Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden, 
      karin.onnheim@gu.se.
FAU - Ekblad, Maria
AU  - Ekblad M
FAU - Gorander, Staffan
AU  - Gorander S
FAU - Lange, Stefan
AU  - Lange S
FAU - Jennische, Eva
AU  - Jennische E
FAU - Bergstrom, Tomas
AU  - Bergstrom T
FAU - Wildt, Sheryl
AU  - Wildt S
FAU - Liljeqvist, Jan-Ake
AU  - Liljeqvist JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150221
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - *Disease Models, Animal
MH  - Female
MH  - Herpes Genitalis/*pathology/*virology
MH  - Herpesvirus 1, Human/*growth & development
MH  - Herpesvirus 2, Human/*growth & development
MH  - Rats
MH  - Survival Analysis
MH  - Vagina/virology
EDAT- 2015/02/24 06:00
MHDA- 2015/06/16 06:00
CRDT- 2015/02/22 06:00
PHST- 2014/10/03 00:00 [received]
PHST- 2015/02/09 00:00 [accepted]
PHST- 2015/02/22 06:00 [entrez]
PHST- 2015/02/24 06:00 [pubmed]
PHST- 2015/06/16 06:00 [medline]
AID - 10.1007/s00705-015-2365-7 [doi]
PST - ppublish
SO  - Arch Virol. 2015 May;160(5):1153-61. doi: 10.1007/s00705-015-2365-7. Epub 2015 
      Feb 21.