PMID- 25702985
OWN - NLM
STAT- MEDLINE
DCOM- 20151130
LR  - 20150223
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 136
DP  - 2015 May
TI  - Determination of hexanal and heptanal in human urine using magnetic solid phase 
      extraction coupled with in-situ derivatization by high performance liquid 
      chromatography.
PG  - 54-9
LID - S0039-9140(15)00008-9 [pii]
LID - 10.1016/j.talanta.2015.01.003 [doi]
AB  - In this study, magnetic solid phase extraction coupled with in-situ 
      derivatization (MSPE-ISD) was established for the determination of hexanal and 
      heptanal in human urine. 2,4-Dinitrophenylhydrazine (DNPH) was used as the 
      derivatization reagent that was adsorbed onto the surface of 
      magnetite/silica/poly(methacrylic acid-co-ethylene glycol dimethacrylate) 
      (Fe3O4/SiO2/P(MAA-co-EGDMA)). And then simultaneous extraction and derivatization 
      of the aldehydes were performed on the DNPH-adsorbed Fe3O4/SiO2/P(MAA-co-EGDMA). 
      The simple, rapid and sensitive determination of hexanal and heptanal can be 
      accomplished within 9min. Under optimized conditions, the limits of detection 
      (LODs) were 1.7 and 2.5nmol/L for hexanal and heptanal, respectively. The 
      relative recoveries ranged from 72.8% to 91.4% with the intra- and inter-day 
      relative standard deviations (RSDs) being less than 9.6%. Furthermore, the 
      proposed method was successfully applied to determine endogenous hexanal and 
      heptanal in human urine from healthy persons and lung cancer patients. The 
      results showed the higher concentrations of hexanal and heptanal were observed in 
      lung cancer patients compared to healthy controls. Thus, the developed MSPE-ISD 
      method is suitable for the determination of aldehydes in urines.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Liu, Jiu-Feng
AU  - Liu JF
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Yuan, Bi-Feng
AU  - Yuan BF
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China. 
      Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150112
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Aldehydes)
RN  - 0 (Phenylhydrazines)
RN  - 0 (Polymethacrylic Acids)
RN  - 1N39KD7QPJ (2,4-dinitrophenylhydrazine)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 92N104S3HF (heptanal)
RN  - 9DC2K31JJQ (n-hexanal)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Aldehydes/chemistry/*urine
MH  - Chromatography, High Pressure Liquid
MH  - Ferrosoferric Oxide/chemistry
MH  - Humans
MH  - Lung Neoplasms/urine
MH  - Magnetic Phenomena
MH  - Phenylhydrazines/chemistry
MH  - Polymethacrylic Acids/chemistry
MH  - Silicon Dioxide/chemistry
MH  - Solid Phase Extraction
OTO - NOTNLM
OT  - Heptanal
OT  - Hexanal
OT  - In-situ derivatization
OT  - Magnetic solid phase extraction
EDAT- 2015/02/24 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/02/24 06:00
PHST- 2014/10/30 00:00 [received]
PHST- 2015/01/01 00:00 [revised]
PHST- 2015/01/03 00:00 [accepted]
PHST- 2015/02/24 06:00 [entrez]
PHST- 2015/02/24 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0039-9140(15)00008-9 [pii]
AID - 10.1016/j.talanta.2015.01.003 [doi]
PST - ppublish
SO  - Talanta. 2015 May;136:54-9. doi: 10.1016/j.talanta.2015.01.003. Epub 2015 Jan 12.