PMID- 2570347 OWN - NLM STAT- MEDLINE DCOM- 19891012 LR - 20190725 IS - 0026-8925 (Print) IS - 0026-8925 (Linking) VI - 217 IP - 2-3 DP - 1989 Jun TI - Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli K12. PG - 281-8 AB - Ribosomal protein S6 of wild-type strains of Escherichia coli contains up to six glutamic acid residues at its C-terminus. The first two residues are encoded by the structural gene for this protein (rpsF) and the rest are added post-translationally. Mutants deficient in this modification were isolated and characterized genetically and biochemically. The S6 protein in these mutants appeared to contain only two glutamic acid residues at the C-terminus as expected. The mutated gene was termed rimK and was mapped at 18.7 min between cmlA and aroA. The rimK gene was cloned into a cosmid vector and its nucleotide sequence determined. Analysis of the transcriptional and translational products of this gene indicates that it encodes a protein with an Mr of 31.5 kDa and that it forms an operon with a gene encoding a 24 kDa protein. An rpsF mutant containing a Glu to Lys replacement in the second residue from the C-terminus of protein S6 was isolated. The S6 protein of this mutant was apparently inaccessible to the RimK modification system. This indicates that the RimK modification system requires the wild-type amino acid sequence at least in the C-terminal region of ribosomal protein S6. FAU - Kang, W K AU - Kang WK AD - Department of Biology, Faculty of Science, Kobe University, Japan. FAU - Icho, T AU - Icho T FAU - Isono, S AU - Isono S FAU - Kitakawa, M AU - Kitakawa M FAU - Isono, K AU - Isono K LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Germany TA - Mol Gen Genet JT - Molecular & general genetics : MGG JID - 0125036 RN - 0 (DNA, Bacterial) RN - 0 (Glutamates) RN - 0 (Ribosomal Protein S6) RN - 0 (Ribosomal Proteins) RN - 3KX376GY7L (Glutamic Acid) SB - IM MH - Amino Acid Sequence MH - Base Sequence MH - Chromosome Mapping MH - Cloning, Molecular MH - DNA, Bacterial/genetics MH - Escherichia coli/*genetics/metabolism MH - *Genes, Bacterial MH - Glutamates/metabolism MH - Glutamic Acid MH - Molecular Sequence Data MH - Mutation MH - Plasmids MH - Protein Biosynthesis MH - Protein Processing, Post-Translational MH - Ribosomal Protein S6 MH - Ribosomal Proteins/*genetics/metabolism MH - Transcription, Genetic EDAT- 1989/06/01 00:00 MHDA- 1989/06/01 00:01 CRDT- 1989/06/01 00:00 PHST- 1989/06/01 00:00 [pubmed] PHST- 1989/06/01 00:01 [medline] PHST- 1989/06/01 00:00 [entrez] AID - 10.1007/BF02464894 [doi] PST - ppublish SO - Mol Gen Genet. 1989 Jun;217(2-3):281-8. doi: 10.1007/BF02464894.