PMID- 25704106
OWN - NLM
STAT- MEDLINE
DCOM- 20160216
LR  - 20150321
IS  - 1873-460X (Electronic)
IS  - 1056-8727 (Linking)
VI  - 29
IP  - 3
DP  - 2015 Apr
TI  - Association of tumor necrosis factor (TNF) promoter polymorphisms with plasma 
      TNF-alpha levels and susceptibility to diabetic nephropathy in North Indian 
      population.
PG  - 338-42
LID - S1056-8727(15)00003-3 [pii]
LID - 10.1016/j.jdiacomp.2015.01.002 [doi]
AB  - AIM: The concept of diabetic nephropathy (DN) as a metabolic disease is now being 
      replaced by chronic low-grade inflammatory disease. Tumor necrosis factor-alpha 
      (TNF-alpha) is a proinflammatory cytokine which plays an important role in the 
      pathogenesis and clinical outcome of DN. Therefore, this work was planned to 
      evaluate the association of -863C/A (rs1800630) and -1031T/C (rs1799964) 
      polymorphisms in TNF gene with plasma TNF-alpha levels and DN among subjects with 
      type 2 diabetes (T2DM) in a population from North India. METHODS: Age and sex 
      matched 100 healthy controls (HC), 100 T2DM subjects without nephropathy (DM) and 
      100 subjects with DN were screened for above polymorphisms using the PCR-RFLP 
      methods. Plasma TNF-alpha levels were measured by ELISA. Analysis of variance and 
      logistic regression were used to associate individual polymorphisms with plasma 
      TNF-alpha levels and DN. RESULTS: The allelic frequencies of -863C/A were 0.86/0.14 
      in HC, 0.72/0.23 in DM and 0.84/0.16 in DN, and that of -1031T/C were 0.89/0.11 
      in HC, 0.95/0.05 in DM and 0.80/0.20 in DN. The carriers of -863A allele had 
      significantly lower plasma TNF-alpha levels (p<0.05). The -863C/A (OR=0.439, 95% 
      CI=0.244-0.789, p=0.006) and -1031T/C (OR=3.0, 95% CI=1.355-6.642, p=0.007) were 
      strongly associated with risk of development of DN. CONCLUSIONS: -863C/A was 
      associated with low whereas -1031T/C with high TNF-alpha levels. The, results suggest 
      that -863C/A polymorphism might be protective whereas -1031T/C may be associated 
      with increased risk for DN in subjects with T2DM from North India.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Gupta, Stuti
AU  - Gupta S
AD  - Molecular Diagnostic Laboratory, Department of Biochemistry, University College 
      of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, 
      Delhi 110095, India.
FAU - Mehndiratta, Mohit
AU  - Mehndiratta M
AD  - Molecular Diagnostic Laboratory, Department of Biochemistry, University College 
      of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, 
      Delhi 110095, India.
FAU - Kalra, Sarathi
AU  - Kalra S
AD  - Department of Medicine, University College of Medical Sciences (University of 
      Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India.
FAU - Kalra, Om P
AU  - Kalra OP
AD  - Department of Medicine, University College of Medical Sciences (University of 
      Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India.
FAU - Shukla, Rimi
AU  - Shukla R
AD  - Molecular Diagnostic Laboratory, Department of Biochemistry, University College 
      of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, 
      Delhi 110095, India.
FAU - Gambhir, Jasvinder K
AU  - Gambhir JK
AD  - Molecular Diagnostic Laboratory, Department of Biochemistry, University College 
      of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, 
      Delhi 110095, India. Electronic address: jassigambhir@yahoo.co.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150109
PL  - United States
TA  - J Diabetes Complications
JT  - Journal of diabetes and its complications
JID - 9204583
RN  - 0 (TNF protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/complications/epidemiology/*genetics
MH  - Diabetic Nephropathies/epidemiology/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - India/epidemiology
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Single Nucleotide
MH  - *Promoter Regions, Genetic
MH  - Tumor Necrosis Factor-alpha/*blood/*genetics
OTO - NOTNLM
OT  - Diabetic nephropathy
OT  - TNF-alpha levels
OT  - Type 2 diabetes mellitus
OT  - -1031T/C polymorphism
OT  - -863C/A polymorphism
EDAT- 2015/02/24 06:00
MHDA- 2016/02/18 06:00
CRDT- 2015/02/24 06:00
PHST- 2014/09/17 00:00 [received]
PHST- 2014/12/09 00:00 [revised]
PHST- 2015/01/05 00:00 [accepted]
PHST- 2015/02/24 06:00 [entrez]
PHST- 2015/02/24 06:00 [pubmed]
PHST- 2016/02/18 06:00 [medline]
AID - S1056-8727(15)00003-3 [pii]
AID - 10.1016/j.jdiacomp.2015.01.002 [doi]
PST - ppublish
SO  - J Diabetes Complications. 2015 Apr;29(3):338-42. doi: 
      10.1016/j.jdiacomp.2015.01.002. Epub 2015 Jan 9.