PMID- 25706387
OWN - NLM
STAT- MEDLINE
DCOM- 20151201
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 2
DP  - 2015
TI  - Integrin-associated protein promotes neuronal differentiation of neural 
      stem/progenitor cells.
PG  - e0116741
LID - 10.1371/journal.pone.0116741 [doi]
LID - e0116741
AB  - Neural stem/progenitor cells (NSPCs) proliferate and differentiate depending on 
      their intrinsic properties and local environment. During the development of the 
      mammalian nervous system, NSPCs generate neurons and glia sequentially. However, 
      little is known about the mechanism that determines the timing of switch from 
      neurogenesis to gliogenesis. In this study, we established a culture system in 
      which the neurogenic potential of NSPCs is decreased in a time-dependent manner, 
      so that short-term-cultured NSPCs differentiate into more neurons compared with 
      long-term-cultured NSPCs. We found that short-term-cultured NSPCs express high 
      levels of integrin-associated protein form 2 (IAP2; so-called CD47) mRNA using 
      differential display analysis. Moreover, IAP2 overexpression in NSPCs induced 
      neuronal differentiation of NSPCs. These findings reveal a novel mechanism by 
      which IAP2 induces neuronal differentiation of NSPCs.
FAU - Fujimura, Kazuhiko
AU  - Fujimura K
AD  - Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto 
      University, Kyoto, Japan.
FAU - Niidome, Tetsuhiro
AU  - Niidome T
AD  - Department of Neuroscience for Drug Discovery, Graduate School of Pharmaceutical 
      Sciences, Kyoto University, Kyoto, Japan.
FAU - Shinozuka, Yoriko
AU  - Shinozuka Y
AD  - Department of Neuroscience for Drug Discovery, Graduate School of Pharmaceutical 
      Sciences, Kyoto University, Kyoto, Japan.
FAU - Izumi, Yasuhiko
AU  - Izumi Y
AD  - Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto 
      University, Kyoto, Japan.
FAU - Kihara, Takeshi
AU  - Kihara T
AD  - Department of Neuroscience for Drug Discovery, Graduate School of Pharmaceutical 
      Sciences, Kyoto University, Kyoto, Japan.
FAU - Sugimoto, Hachiro
AU  - Sugimoto H
AD  - World-Leading Drug Discovery Research Center, Graduate School of Pharmaceutical 
      Sciences, Kyoto University, Kyoto, Japan.
FAU - Akaike, Akinori
AU  - Akaike A
AD  - Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto 
      University, Kyoto, Japan; Graduate School of Pharmaceutical Sciences, Nagoya 
      University, Nagoya, Japan.
FAU - Kume, Toshiaki
AU  - Kume T
AD  - Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto 
      University, Kyoto, Japan.
LA  - eng
PT  - Journal Article
DEP - 20150223
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (CD47 Antigen)
RN  - 0 (CD47 protein, rat)
SB  - IM
MH  - Animals
MH  - CD47 Antigen/*metabolism
MH  - Cells, Cultured
MH  - Neural Stem Cells/*cytology/metabolism
MH  - Neurogenesis/*physiology
MH  - Neurons/*cytology/metabolism
MH  - Rats
MH  - Rats, Wistar
PMC - PMC4338140
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/02/24 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/02/23
CRDT- 2015/02/24 06:00
PHST- 2014/09/04 00:00 [received]
PHST- 2014/12/14 00:00 [accepted]
PHST- 2015/02/24 06:00 [entrez]
PHST- 2015/02/24 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/02/23 00:00 [pmc-release]
AID - PONE-D-14-39733 [pii]
AID - 10.1371/journal.pone.0116741 [doi]
PST - epublish
SO  - PLoS One. 2015 Feb 23;10(2):e0116741. doi: 10.1371/journal.pone.0116741. 
      eCollection 2015.