PMID- 25707992
OWN - NLM
STAT- MEDLINE
DCOM- 20150629
LR  - 20181113
IS  - 2326-5205 (Electronic)
IS  - 2326-5191 (Print)
IS  - 2326-5191 (Linking)
VI  - 67
IP  - 5
DP  - 2015 May
TI  - Articular ankle fracture results in increased synovitis, synovial macrophage 
      infiltration, and synovial fluid concentrations of inflammatory cytokines and 
      chemokines.
PG  - 1234-9
LID - 10.1002/art.39064 [doi]
AB  - OBJECTIVE: The inflammatory response following an articular fracture is thought 
      to play a role in the development of posttraumatic arthritis (PTA) but has not 
      been well characterized. The objective of this study was to characterize the 
      acute inflammatory response, both locally and systemically, in joint synovium, 
      synovial fluid (SF), and serum following articular fracture of the ankle. We 
      hypothesized that intraarticular fracture would alter the synovial environment 
      and lead to increased local and systemic inflammation. METHODS: Synovial tissue 
      biopsy specimens, SF samples, and serum samples were collected from patients with 
      an acute articular ankle fracture (n = 6). Additional samples (normal, ankle 
      osteoarthritis [OA], and knee OA [n = 6 per group]) were included for comparative 
      analyses. Synovial tissue was assessed for synovitis and macrophage count. SF and 
      serum were assessed for cytokines (interferon-gamma [IFNgamma], interleukin-1beta [IL-1beta], 
      IL-6, IL-8, IL-10, IL-12p70, and tumor necrosis factor alpha) and chemokines 
      (eotaxin, eotaxin 3, IFNgamma-inducible 10-kd protein, monocyte chemotactic protein 1 
      [MCP-1], MCP-4, macrophage-derived chemokine, macrophage inflammatory protein 1beta, 
      and thymus and activation-regulated chemokine). RESULTS: Synovitis scores were 
      significantly higher in ankle fracture tissue compared with normal ankle tissue 
      (P = 0.007), and there was a trend toward an increased abundance of CD68+ 
      macrophages in ankle fracture synovium compared with normal knee synovium 
      (P = 0.06). The concentrations of all cytokines and chemokines were elevated in 
      the SF of patients with ankle fracture compared with those in SF from OA patients 
      with no history of trauma. Only the concentration of IL-6 was significantly 
      increased in the serum of patients with ankle fracture compared with normal serum 
      (P = 0.027). CONCLUSION: Articular fracture of the ankle increased acute local 
      inflammation, as indicated by increased synovitis, increased macrophage 
      infiltration into synovial tissue, and increased SF concentrations of biomarkers 
      of inflammation. Characterizing the acute response to articular fracture provides 
      insight into the healing process and may help to identify patients who may be at 
      greater risk of PTA.
CI  - (c) 2015, American College of Rheumatology.
FAU - Furman, Bridgette D
AU  - Furman BD
AD  - Duke University Medical Center, Durham, North Carolina.
FAU - Kimmerling, Kelly A
AU  - Kimmerling KA
FAU - Zura, Robert D
AU  - Zura RD
FAU - Reilly, Rachel M
AU  - Reilly RM
FAU - Zlowodzki, Michal P
AU  - Zlowodzki MP
FAU - Huebner, Janet L
AU  - Huebner JL
FAU - Kraus, Virginia B
AU  - Kraus VB
FAU - Guilak, Farshid
AU  - Guilak F
FAU - Olson, Steven A
AU  - Olson SA
LA  - eng
GR  - AR-48852/AR/NIAMS NIH HHS/United States
GR  - R01 AR048852/AR/NIAMS NIH HHS/United States
GR  - AG-15768/AG/NIA NIH HHS/United States
GR  - R01 AG046927/AG/NIA NIH HHS/United States
GR  - R01 AR048182/AR/NIAMS NIH HHS/United States
GR  - AG-46927/AG/NIA NIH HHS/United States
GR  - P01 AR050245/AR/NIAMS NIH HHS/United States
GR  - R01 AG015768/AG/NIA NIH HHS/United States
GR  - AR-48182/AR/NIAMS NIH HHS/United States
GR  - AR-50245/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheumatol
JT  - Arthritis & rheumatology (Hoboken, N.J.)
JID - 101623795
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Ankle Fractures/*immunology
MH  - Case-Control Studies
MH  - Chemokines/*immunology
MH  - Cytokines/immunology
MH  - Female
MH  - Humans
MH  - Inflammation Mediators
MH  - Macrophages/*immunology
MH  - Male
MH  - Middle Aged
MH  - Osteoarthritis/immunology
MH  - Osteoarthritis, Knee/immunology
MH  - Synovial Fluid/*immunology
MH  - Synovial Membrane/cytology/*immunology
MH  - Synovitis/*immunology
MH  - Young Adult
PMC - PMC4819001
MID - NIHMS761656
EDAT- 2015/02/25 06:00
MHDA- 2015/06/30 06:00
PMCR- 2016/04/03
CRDT- 2015/02/25 06:00
PHST- 2014/10/31 00:00 [received]
PHST- 2015/02/03 00:00 [accepted]
PHST- 2015/02/25 06:00 [entrez]
PHST- 2015/02/25 06:00 [pubmed]
PHST- 2015/06/30 06:00 [medline]
PHST- 2016/04/03 00:00 [pmc-release]
AID - 10.1002/art.39064 [doi]
PST - ppublish
SO  - Arthritis Rheumatol. 2015 May;67(5):1234-9. doi: 10.1002/art.39064.