PMID- 25708631
OWN - NLM
STAT- MEDLINE
DCOM- 20151028
LR  - 20150224
IS  - 1559-4106 (Electronic)
IS  - 1559-4106 (Linking)
VI  - 10
IP  - 1
DP  - 2015 Mar 10
TI  - Latest applications of 3D ToF-SIMS bio-imaging.
PG  - 018902
LID - 10.1116/1.4907727 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a rapidly developing 
      technique for the characterization of a wide range of materials. Recently, 
      advances in instrumentation and sample preparation approaches have provided the 
      ability to perform 3D molecular imaging experiments. Polyatomic ion beams, such 
      as C60, and gas cluster ion beams, often Arn (n = 500-4000), substantially reduce 
      the subsurface damage accumulation associated with continued bombardment of 
      organic samples with atomic beams. In this review, the capabilities of the 
      technique are discussed and examples of the 3D imaging approach for the analysis 
      of model membrane systems, plant single cell, and tissue samples are presented. 
      Ongoing challenges for 3D ToF-SIMS imaging are also discussed along with recent 
      developments that might offer improved 3D imaging prospects in the near future.
FAU - Fletcher, John S
AU  - Fletcher JS
AD  - Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 
      96 Gothenburg, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20150310
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
SB  - IM
MH  - Animals
MH  - Histocytochemistry/methods
MH  - Imaging, Three-Dimensional/*methods/trends
MH  - Plants
MH  - Spectrometry, Mass, Secondary Ion/*methods/trends
EDAT- 2015/02/25 06:00
MHDA- 2015/10/29 06:00
CRDT- 2015/02/25 06:00
PHST- 2015/02/25 06:00 [entrez]
PHST- 2015/02/25 06:00 [pubmed]
PHST- 2015/10/29 06:00 [medline]
AID - 10.1116/1.4907727 [doi]
PST - epublish
SO  - Biointerphases. 2015 Mar 10;10(1):018902. doi: 10.1116/1.4907727.